CROP PROPagation
CROP PROPagation
Uni
t                                Plant Propagation
INTRODUCTION
Plant propagation, in simple words, may be
defined as multiplication or reproduction of plants.
Commercialisation of crops leads to the development
of various techniques and procedures of plant
propagation. Each technique has its own merits and
demerits.
    Each plant responds differently to different
methods of propagation. Various techniques of
propagation have been developed with the objective to
have uniformity in crops, early bearing, increased
production, resistance against pests and diseases, and
introduce certain characters in new generation. These
objectives have made plant propagation interesting
and challenging.
    Propagation of plant is the involvement of science
and art in a skillful way. Basic knowledge and skill of
it can be a better source of income through commercial
nurseries. It helps in maintaining the plant stock and
preserving endangered (extinct) species.
    Plants can be propagated by sexual and asexual
means. Sexual means includes propagation by seeds,
     NOTES   while asexual propagation is based on the utilisation of
             vegetative parts of plants for raising new ones.
             Vegetative parts of plants like shoots, leaves, roots,
             stem, buds and underground parts are used in
             different ways for reproducing new plants. The most
             common asexual propagation methods include cutting,
             layering, grafting and budding, which need specialised
             skill and are done differently in different plants.
                 Growing of tissues in controlled conditions is an
             advance and recent method of vegetative propagation.
             It is known as ‘tissue culture’. It is a highly specialised
             technique of propagation. By using this technique, a
             large number of true-to-type virus-free saplings can be
             produced in a short span.
                                 What is a seed?
              A seed is a ripened ovule developed after fertilisation.
              It consists of an ‘embryo’ and stored food material,
              both of which are enclosed in a special covering
              known as ‘seed coat’. Plants germinate from
              seeds when they are provided with favourable
              growing conditions.
             TYPES OF PROPAGATION
             Sexual propagation
             Propagation or multiplication of plants by seeds is
             known as ‘sexual propagation’. Seeds are formed as
             a result of successful fertilisation and combination of
             parental gametes. It is an old and easy method and is
             widely used for the propagation of crops like ornamental
             annuals, vegetables, medicinal and fruit plants, such
             as papaya.
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                                                      GARDENER – CLASS XI
      •   Some plants like papaya, marigold, chilli,                NOTES
          capsicum, tomato, etc., cannot be propagated by
          asexual method.
      •   It is the only means of creating genetic diversity
          of plants.
      •   New varieties and cultivars of ornamental
          and vegetable crops can be developed only by
          this method.
      •   A large number of rootstocks for budding and
          grafting purpose is also raised by this method.
      •   Seeds can be transported easily and stored for a
          longer time using this method.
Asexual propagation
It is also called ‘vegetative propagation’. The vegetative parts
     of a plant like leaf, stem, root or their modified forms are
     used for propagation. Most of the horticultural crops are
     commercially propagated by vegetative or asexual
     method of propagation.
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   PLANT PROPAGATION
     NOTES     •   Plants propagated by asexual propagation are
                   true-to-type genetically.
               •   By top working (using budding and grafting), old
                   and economically low productive fruit plants can
                   be converted into superior ones.
               •   Advantages offered by rootstocks and scion can be
                   exploited through asexual method.
               •   Maturity is uniform and the plant gives quality yield.
               •   Plants propagated by asexual method are
                   small in size, so spraying of chemicals and
                   harvesting are easy.
               •   This method enables noble plant production, e.g.,
                   different colours of flowers in a single rose plant
                   and different types of mangoes in one mango plant
                   can be produced through asexual method only.
             Stem cutting
             Based on the age and maturity of shoots detached for
             vegetative propagation, stem cuttings is of four types.
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                                                       GARDENER – CLASS XI
     (i) Hardwood cutting
    (ii) Semi-hardwood cutting
   (iii) Softwood cutting
   (iv) Herbaceous cutting
Hardwood cutting
Such a cutting is taken from woody plants.
Mostly, deciduous plants are propagated by
this method. One-year old mature branch is
cut into pieces of suitable sizes and planted
in the rooting medium, e.g., rose, grapes, fig,
pomegranate, bougainvillea,tabernaemontana,
lagerstroemia, jasminum, hibiscus, etc.
Procedure
  • Select branches of one-year old
     healthy plants, having pencil thickness.
     Cut the branches into 10–15 cm long               Fig. 3.1: Hardwood cuttings
     cuttings.
  • Long cuttings are used to raise rootstocks for
     fruit trees. Each cutting must have at least 4–5
     dormant vegetative buds. Leaves and thorns,
     if present, are completely removed. This checks
     transpiration loss.
  • A slanting cut is given at the base of the cuttings
     just below the node and a straight upper cut is
     given away from the top bud.
  • The cut portion will help identify the planting
     position. Slanting cut at the base is given so that
     a large area of the cuttings is in contact with the
     rooting medium for inducing roots.
  • The secretion of hormones at the bud near the
     cut portion induces rooting. Straight cut at upper
     end reduces transpiration loss, which can be
     inhibited by the application of wax.
  • The cuttings are planted slant-wise in a nursery
     bed or small poly bags for growing plants. Callus
     tissues form the cambium layer and rooting
     takes place in this region. The best season for
     planting the cuttings is monsoon for evergreen
     plants and November–February for deciduous
     plants. Cuttings can be planted in greenhouse or
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PLANT PROPAGATION
poly-house for better results.
                                 GARDENER – CLASS XI
                                         Semi-hardwood cutting
                                            A semi-hardwood cutting is taken from
                                            4 to 9-month old shoots of current season
                                            woody plants. Most ornamental foliage
                                            plants like croton, acalyphas, aralias,
                                            diffenbachia, russelia, cestrum, nerium, etc.,
                                            are propagated by semi-hardwood cuttings.
                                            Procedure
                                        Semi-hardwood cuttings are prepared from
                                            branches having pencil thickness. The
                                            length of these cuttings varies from 7.5 to
                                            15 cm. The cuttings must have at least 4–5
                                            dormant vegetative buds. Some leaves are
                                            retained as they help in preparing food by
     Fig. 3.2: Semi-hardwood cuttings       photosynthesis. Large leaves are reduced
                                            in size by cutting. A slant basal cut is
                               given just near the vegetative bud and a straight top
                               cut must be given away from the bud. The slant cut
                               helps to expose more area of the cambium layer, which
                               helps in more water absorption and callus formation.
                               The upper straight cut minimises exposure to the
                               atmosphere, which reduces transpiration loss from the
                               cuttings. It is useful to dip the top of the cuttings in
                               wax to check transpiration and infections. Dipping the
                                                    base of the cuttings before
                                                    planting in IBA @ 5000 ppm
                                                    induces early rooting. The cuttings
                                                    are planted in slanting position so
                                                    that their maximum base is in
                                                    contact with the rooting medium.
                                                    The planting season for semi-
                                                    hardwood cuttings is monsoon.
                                                    Commercially, such cuttings are
                                                    rooted under mist spray or fog.
                                                     Softwood cutting
                                                     Such a cutting is taken from
           Fig. 3.3: Softwood cuttings           herbaceous or succulent plants.
                                                 Shoots of 2 to 3-month old plants
                             are selected for softwood cuttings. Examples are
                             alternanthera, coleus, duranta, clerodendrum, etc.
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                                                                        GARDENER – CLASS XI
Procedure
Softwood cuttings are prepared from tender but
mature branches. The length of these cuttings varies
from 10–12 cm. Tender shoots do not have sufficient
food material. Hence, all leaves present on the shoots
are retained for photosynthesis. The cutting material
are gathered early in the morning and must be kept
moist by keeping them in a wet cloth. Sandy loam
medium is the best for planting softwood cuttings.
Herbaceous cutting
Such a cutting is taken from herbaceous plants. Shoots
of 1 to 2-month old plants are selected for herbaceous
cuttings. Examples are chrysanthemum, iresine, pilea,
dahlia, petunia, carnation, marigold, etc.
Procedure
Herbaceous cuttings are made from tender
succulents, especially the leafy part of the
stems of herbaceous plants. Terminal,
measuring 8–12 cm, of a healthy shoot
is cut and the basal leaves are removed,
leaving the upper leaves undisturbed. The
cuttings once detached must not desiccate
at the cut and are rooted well under mist.
The application of auxins promotes the
regeneration of adventitious roots. Sandy
loam medium is the best for planting
herbaceous cuttings.
Selection of cutting
Plants with thick fleshy leaves having
buds are propagated by leaf cutting.
Vegetative buds are present in the notches
of leaf margin (bryophyllum) or on the vein
(begonia rex). Leaf blade or pieces of it
with bud are put on the rooting medium
under favourable conditions. In case of
black raspberry, the leaf blade, along with
petiole and a short piece of the stem with
                                               Fig. 3.5: Propagation through leaf (bryophyllum)
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PLANT PROPAGATION
     NOTES   attached axillary buds, are kept in the medium for
             rooting. Plants like snake plant (senseveria), blackberry,
             rhododendron and bryophyllum are propagated by this
             method.
                                Practical Exercise
                                          Activity
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                                                           GARDENER – CLASS XI
   6.   Sexually propagated plants show                 .
                                                                NOTES
   7.   Plants propagated through seeds have
        juvenile phase.
   8.   Vegetative propagation is also called
        propagation.
   9.   Bougainvillea is propagated by              .
   1.   Bryophyllum is propagated by             .
        (a) root cutting       (b) stem cutting
        (c) leaf cutting       (d) seeds
   2.   Jasminum is propagated by                   .
        (a) root                       (b) stem
        (c) leaf                       (d) seed
   3.   Asexually propagated plants                     .
        (a) are true-to-type           (b) bears late fruit
        (c) live longer                (d) have large canopy
   4.   Hardwood cutting is, generally, used in
        branch.
        (a) one-year old               (b) two-year old
        (c) three-year old             (d) four-year old
C. Subjective Questions
A B
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PLANT PROPAGATION
                               SESSION 2: PLANT PROPAGATION BY LAYERING
                               Layering
                               It is an attached method of propagation. In this
                               method, roots are allowed to develop on the covered
                               portion of the stem while still being attached to the
                               mother plant. After the emergence and development of
                               the roots, this portion is separated from the mother
                               plant and allowed to grow as a new plant on its own
                               root stem. Such root stem is known as ‘layer’.
                               Types of layering
                                     (i) Simple layering
                                    (ii) Compound or serpentine layering
                                   (iii) Trench layering
                                   (iv) Mound layering or stooling
                                    (v) Air layering
                               Simple layering
                               In simple layering, a partial tongue-like cut is given on
                               a branch. The branch is then bent to the ground and
                               the treated portion is covered with soil, keeping the
                               top or terminal portion exposed. The layered branches
                               produce roots in weeks and are ready for transplanting in
                               a nursery after detaching them carefully. Examples
                               are jasmine,
                                             ixora, clerodendron, pyrostegia, etc.
                                              Procedure
                                              Select one-year old healthy, flexible,
           (a) Selected branch                long un-branched shoot near the ground
                                              level. Remove leaves of the selected
                                              shoot, retaining some at the top. The
                                              retained leaves prepare food through
                                              photosynthesis. Bend down the shoot so
                                              that some part of it touches the ground.
     (b) Slanting cut given on branch
                                              At that portion, generally, 15−30 cm away
                                              from the terminal end, a sharp slanting
                                              inward cut of 2−3 cm is given. A small
                                              matchstick is inserted in the cut to keep
                                              the slit open. Bend down the branch and
     (c) Cut portion covered with soil        cover the cut part with soil. Keep some
                                              weight or stone over the buried part so
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                                                                       GARDENER – CLASS XI
          Fig. 3.6 (a–c): Simple   that it is not pulled upward, and remains
          layering
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                                                                       6
PLANT PROPAGATION
in the same position. A stake is fixed near the layered
branch and the branch operated upon is tied with it.
Water the layered portion regularly. After 3−4 weeks,
rooting starts at the operated portion and this can be
indicated by sprouting buds on the shoot. After this,
the layer is separated from the mother plant and planted
in a new place.
Compound or serpentine layering
Compound layering is similar to simple layering, except
the branches are alternately covered and exposed
along their length. The branches must
be longer so that they can be layered at
several places. This method is followed
in plants like bougainvillea, jasmine,
clematis, muscadine grape and wisteria.
                                                    (a) Circular cut or girdling on branch
Procedure
One-year old healthy and flexible long
shoot near the ground is selected for
compound layering. The selected stem is
placed in soil in a way that the nodes at
                                                       (b) Cut portion covered with soil
certain distance are covered under the
soil and the intermediate internodes are
exposed. Remove leaves from the selected
branch but retain few leaves at the top.
Give two circular cut around the bark
about 2.5−4 cm wide. Remove the bark of                (c) Branch prepared for planting
the operated portion (girdling). Apply Fig. 3.7 (a–c): Compound or serpentine layering
rooting hormone
to the girdled portion and cover it with soil. The same
branch is operated at 3−4 places at certain distance in
the same way. The growing shoots, which emerge from
the covered portion of the branch, are separated from the
mother plant for planting in a nursery.
Trench layering
Trench layering is primarily used in fruit plants.
Covering the shoots with soil results in etiolation, so it
is also known as ‘etiolation layering’. New shoots arise
from the length of the burried branches. After rooting,
individual shoots are separated from the mother plant.
This method is followed in apple, cherry, pear, jasmine
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                                                                            GARDENER – CLASS XI
and rhododendron.
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                    6
PLANT PROPAGATION
           Fig. 3.8: Trench layering
     Procedure
     One-year old healthy and flexible long shoot near the
     ground is selected. The selected stem is placed in a
     shallow trench in a way that the middle portion of it is
     buried. Remove leaves from the branch but retain few
     leaves at the top. Cover the whole branch with moist soil
     5–10 cm deep. The terminal portion is left exposed to
     manufacture food and hormones for the developing
     plants. After some weeks, shoots arise from the nodes,
     which are covered by soil. The covering of the shoots
     with soil results in etiolation of the shoots and helps in
     rooting. Individual shoots with roots (layers) are
     separated from the mother plant and planted in a
     nursery.
     Mound layering or stooling
     This method is followed in plants whose branches are
     firm and difficult to bend. The selected plant must be at
     dormant stage at the time of layering.
     Procedure
     Cut back the upper portion of the plant 2.5 cm above
     the ground level. After few days, new shoots will emerge.
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                                              GARDENER – CLASS XI
                    When the shoots grow to a height of 7−15 cm and
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                                                               6
PLANT PROPAGATION
   become little sturdy, place loose soil around them so
   that they are half buried. When the shoots attain a
   height of 20−25 cm, again add soil around them so that
   they are half buried. Water the heaped soil regularly. It
   will take 3−4 months to get the layers. Cut the rooted
   layers close to the base from the mother plant and plant
   it in a nursery. Examples are apple, guava, currant,
   gooseberry, pear, etc.
    (a) Mother
       plant       (b) Top      (c) Soil is put    (d) Growth of new              (e) Rooted layers
                  removed        around the         shoots increase                 are detached
                  from the     stump as new
                 mother pant   shoots emerge
                                   Fig. 3.9 (a – e): Mound layering or stooling
   Air layering
   It is also known as ‘gootee’. Examples are Ficus
   elastica, Callistemon, croton, monstera, citrus fruits,
   lychee, philodendron, pomegranate, etc.
    Procedure
Select healthy, vigorously growing aerial branch having
    pencil-size thickness. The selected branch must be of
    the past growing season. Girdle the selected branch up to
    2−3.5 cm wide just below the node 15–30 cm back from
    the tip of the shoot. A strip of the bark from the girdled
    portion is removed. Scrap the girdled portion, which
    helps in the removal of phloem tissues and prevents
    formation of bark at the girdled portion. Excessive
    moisture from sphagnum moss is squeezed out before
    placing it over the cut portion. A piece of polyethylene
    film is carefully wrapped around the branch so that
    the sphagnum moss is completely covered. Both the
    ends of the polyethylene film are made airtight by tying
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   PLANT PROPAGATION
     NOTES   them with strings. The layer is removed from the parent
             plant when roots are observed through the transparent
             polyethylene film. It takes 2−3 months for rooting. Rainy
             season is the best for air layering.
                                 Practical Exercise
                                           Activity
               Demonstrate simple layering.
               Material required: Sharp knife, stone pieces or hooks or
               pegs, polythene bags, secateurs and matchstick
               Procedure
                 • Select one-year old healthy and flexible
                     long un-branched shoots near the ground
                     level.
                 • At a distance of 15−30 cm back from the tip,
                     make a sharp slanting inward cut and insert
                     a matchstick.
                 • Bend the shoot gently to the ground so that the cut
                     part can be inserted into the soil.
                 • Cover the rooting region with soil.
                 • Keep a stone on the part covered with soil in order to
                     retain the layer in place.
                 • Drive a vertical stake to the soil by the side of the
                     layered branch.
                 • Tie the branch to the stake with a gunny thread.
                 • Water the layered portion regularly till rooting starts.
             Check Your Progress
              A. Fill in the Blanks
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                                                              GARDENER – CLASS XI
      3.     In trench layering, the whole branch buried in soil is   NOTES
             up to              cm deep.
             (a) 1−2                 (b) 3−4
             (c) 5−10                (d) 12−15
      4.     The same branch is operated at 3−4 places at
             certain distance in                     layering.
             (a) trench              (b) simple
             (c) air                 (d) compound
 C. Subjective Questions
    1. Write the procedure of compound layering.
      2.     Discuss in detail the process of air layering.
                  A                                    B
           1. Air layering                (a) Plant must be at
                                              dormant stage
           2. Circular removal of bark    (b) Apple
           3. Mound layering              (c) Girdling
           4. Trench layering             (d) Serpentine layering
           5. Compound layering           (e) Gootee
Advantages of grafting
  •         Plants propagated by grafting are true-to-type,
            and bear flowers and fruits early.
  •         The plants can be multiplied and preserved
            by grafting.
  •         Local variety of older plants can be improved to
            superior variety by top working.
  •         Wounded or damaged tree trunks can be repaired
            by special grafting methods.
  •         Rootstock has an influence on resistance, vigour
            and quality of grafted plants.
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PLANT PROPAGATION
     NOTES        •   Certain rootstocks, which are tolerant to saline
                      and alkaline soils and other adverse conditions,
                      can be used for grafting.
             Disadvantages of grafting
                  •   It requires specialised skill.
                  •   It is an expensive method of propagation.
                  •   New varieties cannot be developed by grafting.
                  •   Plants produced through grafting are short lived
                      as compared to plants propagated by seeds.
                  •   When contaminated tools or propagation material
                      are used in grafting, newly propagated plants may
                      also get infected.
             Rootstock
             The part of the graft that provides root system to the
                grafted plant is known as ‘rootstock’. It is, normally,
                raised by seeds in the seedbed, and then, transplanted
                in the nursery bed for budding and grafting.
                Rootstocks are also raised in pots and polythene bags.
             Characteristics of rootstock
                  •   Adaptable to local climatic conditions
                  •   Resistant to adverse climatic and soil conditions
                  •   Resistant or tolerant to pests and diseases
                  •   Propagates easily
                  •   Compatible with scion
                  •   Promotes early healing and formation of
                      cambium layer
             Raising of rootstock
             Generally, rootstocks are raised by seeds (mango and
                citrus fruits), or sometimes, by cuttings (rose). Seeds
                are sown or cuttings are planted on raised beds or in
                poly bags for raising rootstocks. After the germination
                of seeds or rootings of cuttings, the seedling rootstocks
                are transplanted in poly bags or nursery beds. Once they
                reach the stage of growth, they are used as rootstocks
                for grafting or budding. Sometimes, the rootstocks are
                not of the same species, e.g., for grapes (Vitis vinifera),
                the rootstock used is a related species Vitis berlandieri.
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                                                         GARDENER – CLASS XI
Scion                                                         NOTES
The upper portion of graft combination taken from the
desired plant to be multiplied is known as ‘scion’.
Characteristics of scion
  •   Scion wood must be of the previous season but
      not from more than one-year old plant.
  •   Flowering shoots or shoots from where the
      harvesting is recently done must be avoided.
  •   Healthy and well-developed vegetative buds must
      be selected.
  •   The scion or bud sticks must be selected from
      known performing orchard trees.
Selection of scion
  •   The mother plant must be vigorous, high
      yielding, true-to-type and free from undesirable
      bud mutation and viral diseases.
  •   It is advisable to collect scion from grown-up trees.
  •   It must be preconditioned by defoliating the
      branch before it is used for budding or grafting.
      Defoliating helps the buds to swell.
Methods of grafting
Grafting methods can be grouped into the following.
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PLANT PROPAGATION
                independent self-sustaining plants are grafted together.
                After the successful union of the graft, the scion plant
                is detached below the graft union from the mother plant
                and the top of the rootstock plant is removed above
                the graft. This method is useful for plants, in which
                successful graft unions are difficult to obtain. This
                method is, usually, performed for plants growing in a
                container, as well as, big trees. In the latter case, the
                rootstock seedling is brought near the scion branch by
                erecting a platform.
                Selection of grafting material
                Approach grafting can be done in two ways, and
                accordingly, their names are given as ‘sliced approach
                grafting’ and ‘tongue approach grafting’. In both
                the methods, the success of grafting depends on the
                                               thickness of the scion and
                                               rootstock. Both must be
                                               compatible             and
                                               comfortable at the union.
                                               The rootstock and scion
                                               must be of almost the
                                               same thickness. Select the
                                               scion branch on the
                                               mother plant of desired
                                               characteristics.
     c                                           Sliced approach grafting
                d
                                                     Procedure
                                                      • Bring the selected
                                      a                   rootstock and scion
b                                                         close together.
                                                      • Find out the most
                                                          comfortable point of
                           (a) Scion                      contact.
                           (b) Rootstock              • At the point of contact,
                           (c) Point of stock to cut      a thin slice of wood
                           (d) Point of scion to cut
                                                          along with a 2.5 to
                                                          5-cm long bark from
                                                          the rootstock and the
 Fig. 3.10: Approach grafting (inarching)
                                                      scion is removed.
                       • The operated size must be uniform on both the
                             stems of the rootstock and the scion.
                                                             GARDENER – CLASS XI
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PLANT PROPAGATION
     •    The cut surfaces are then brought together so that
          they cover each other completely by overlapping.
          Press them firmly together and tie them with a
          waxed string or polythene tape, so that water does
          not enter.
     •    After successful union, head back the rootstock
          above the union and cut the scion below the
          union, e.g., mango, guava, sapota, etc.
   Tongue grafting
   This method differs from the former as cuts are given on
   both the scion and rootstock.
Procedure
     • Bring the selected rootstock and scion close
        together.
     • Find out the most comfortable point of contact.
     • Remove a slice of wood along with a 2.5 to 5-cm
        long bark from the rootstock and scion.
                                                                                GARDENER – CLASS XI
         NOTES           •   A second slanting partial cut downward on the
                             stock and upward on the scion is made, producing
                             a thin tongue-like structure of the same size on
                             the stem of the stock and the scion.
                         •   Insert the scion in the stock so that these tongue
                             cuts interlock.
                         •   All operated portions must be in contact with
                             each other.
                         •   Tie the operated portions.
                   Scion detached method
                   This method is a more popular method of grafting and
                      comparatively easier to perform. Besides, the rate of
                      success of plant propagation is more in this case. In
                      this method, the scion is first detached from the mother
                      plant, and then, inserted or tied on the rootstock. The
                      types of scion detached method are:
                        • Veneer grafting
                        • Side grafting
                        • Wedge or cleft grafting
                        • Stone or epicotyl grafting
                        • Whip or splice grafting
                        • Bark grafting
                   Veneer grafting
                   It is a simple and economical method of grafting. It the most
                        ideal method for establishing in situ orchards and top
                        working of old unproductive orchards. The best time
                        in north India for veneer grafting is March−April and
                        July−August.      Mango,    cashew       and     peach      are
                        commercially propagated by this technique. Veneer
                        grafting differs from side grafting. In this, the vertical flap
                        of the stock is completely removed and a slanting cut is
                        given on one side of the scion.
                       Procedure
                         • A shallow 3 to 5-cm long downward cut is made
                            on the selected rootstock.
                         • At the base of the first cut, a short inward and
                            downward cut is made that intersects the first
                            cut.
                         • In between both the cuts, remove the piece of
                            wood along with the bark by making a small
                            notch in the rootstock.
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                                                                                  7
PLANT
  70 PROPAGATION
  •     The scion is operated with a matching long cut
        on one side and a short cut on the opposite side
        is given at the base.
  •     Insert the scion and fix it in the rootstock. Care
        must be taken to ensure that the cambium layer
        matches at least one side of the cut surface.
  •     Wrap and tie the scion and rootstock firmly.
  •     Cut back the rootstock above the union after
        successful union.
  •     This method is used for grafting conifers,
        deciduous trees and shrubs.
      (a) Prepared   (b) Prepared    (c) The scion is          (d) Wrapped scoin             (e) Prepared
          stock          scion    inserted in the stock            and stock                     plant
                                     Fig. 3.12 (a–e): Veneer grafting
Side grafting
In this method, the operated scion is inserted
into the side of the established rootstock,
which has more girth than the scion,
e.g., hibiscus.
Selection of material
  • A rootstock of 2.5 cm diameter is selected.
  • The scion needs to have 3–5 buds and
      must be about 7.5 cm long.
  • The scion must be comparatively
                                                                        (a)                          (b)
      thinner than the rootstock.
Procedure                                                                Fig. 3.13 (a–b): Side grafting
                       Procedure
                         • The rootstocks of required plant species are raised
                            in poly bags.
                         • The seedling of the suitable rootstock, which is
                            4 to 5–month old is selected.
                         • Head back the rootstock.
                         • A sharp vertical downward cut of 3−5 cm is made
                            in the centre of the stem.
                         • Two slanting cuts of the same length (3–5 cm) as
                            in the rootstock are given on the sides towards the
                            base on the scion shoot.
                         • This will give a wedge-shaped appearance to the
                            scion stick.
                         • The wedge-shaped scion is inserted in the split of
                            the rootstock.
                         • Insert the scion in a way that it matches the
                            cambium layer at least on one side with the
  72
                            stock.
  72                                                                          71
                                                                              7
PLANT PROPAGATION
     •       Tie the grafted portion firmly in position with a
             polythene tape.
     •       After successful union, the terminal buds of the
             scion begin to sprout.
     •       Loosen or remove the polythene tape to allow the
             shoot to grow normally.
     •       It is better to stake the newly grafted plant.
                                                                                  (e)
                     (b) The split stock   (c)                                          Wrapped
   (a)                                                      (d) Scion inserted                             (f)
                        held open with                                                  scion and
         Selected       the help of              Prepared                               rootstock                Prepared
         rootstock      a chisel                               in the rootstock                                  plant
                                                                                                    GARDENER – CLASS XI
         NOTES         Procedure
                         • A wedge in the scion is made at the base by
                            giving two slanting cuts of 5 cm.
                         • Head back the stock by giving a straight
                            horizontal cut.
                         • From the centre of the stock, give a 5-cm long
                            vertical cut downward.
                         • Insert the wedge-shaped scion in the split portion of
                            the stock so that the operated portion is in full
                            contact.
                         • Tie the graft firmly with a polythene strip. The
                            successful graft sprouts and new shoots emerge.
                            Such grafts are ready for planting within one year,
                            e.g., mango.
             Bridge grafting
             This method is used for repairing wounds in trees made
                by implements, frost, rodents or diseases. In this
                grafting, the bark of a tree is damaged, resulting into
                girdling. A completely girdled tree will die. Bridge
                grafting repairs girdling.
             Selection of material
                  • The rootstock must be in sap-flowing condition.
                  • The scion comprises one-year old dormant shoots
                      of 6−12 mm in diameter.
                  • The number of scion sticks depends upon the size
                      of the wound to be repaired.
                  • The selected scion may be of the same or a
                      compatible plant.
             Procedure
                  • Trim the wounded area by removing the dead bark.
                  • The cuts are made in the bark at the top and
                     bottom of the wound at 5 to 7.5 cm distance.
                  • Long slanting cuts are given on the scion at the
                     top, as well as, bottom.
76
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                                                        GARDENER – CLASS XI
  •  Both the cuts must be on the same side.
  •  A sharp wedge of scion is made by an additional
     short, slanting cut opposite to the first.
  • Prepare the required number of scions in the
     same way.
  • Buds on the scion(s) are removed.
  • The operated portion
     of    the    scion    is
     inserted in each slot
     of the bark on the
     rootstock in a way
     that     the     wedge
     remains under the
     flap of the bark at
  each end.                         (a)             (b)               (c)             (d)
  • The scions must be         Remove the       Prepared          Prepared        Insert the
     put in upright position. bark from the     rootstock           scion         scion into
                                rootstock                                       the prepared
     The graft unions at top                                                      rootstock
     to bottom are waxed.                    Fig. 3.16 (a–d): Bridge grafting
Top working
Top working is a method of grafting by which inferior or
older plants are rejuvenated into superior or new ones.
Top working is, generally, adopted in plants with long
leaves. It is suitable for plants like apple, avocado, citrus
fruits and vegetable, mango and shrubs or vines. Top
working can be done by top grafting or top budding. For
top working, cleft, whip, wedge or side grafting methods
can be used, according to the suitability of a plant. Top
working is, usually, done during spring.
Selection of material
  • Prepare the rootstock and scion by any of the
     above suitable method.
  • Usually, 3–5 scaffold branches must be used for
     rejuvenation in top working.
  • In frame working, the secondary scaffold
     branches are used for grafting.
  • The branches to be worked with must be
     well-distributed around the trunk.
  • The branches must be 3−10 cm in thickness.
  • Scion sticks of the desired cultivar with 7−10
     dormant buds are selected.
                                                                                        75
                                                                                        77
PLANT PROPAGATION
     NOTES   Precautions
                  • Observe the progress of the branches of a top
                     worked tree every 3−5 days.
                  • Cracks developed in the wax coating must be
                     re-waxed.
                  • Whitewash the trunk to avoid sunburn.
                  • The scion must also be protected from the Sun by
                     keeping the graft in shade.
                  • New shoots developing from scions are tied to
                     stakes to avoid breaking off due to winds.
                  • New growth on older branches and trunk must
                     be removed from time-to-time.
                  • The top worked trees must be regularly irrigated
                     and manured.
                               Practical Exercises
                                       Activity 1
               Demonstrate veneer grafting.
               Material required: Rootstock and scion of mango plant,
               grafting knife and grafting tape
               Procedure
                 •   Select a one-year old healthy rootstock of a mango
                     plant.
                 •   Select the scion of desired variety having 3–5 buds
                     and about 7.5 cm in length.
                 •   Make 2.5 to 5-cm long shallow cut on the rootstock
                     downwards, and at the base of the first cut, a short
                     inward cut is made. Then, remove the bark and wood.
                 •   Make a cut of the same size on the scion and a very
                     short cut at the base of the scion opposite to the
                     long cut.
                 •   Insert the scion and fix it into the stock.
                 •   Wrap and tie the grafted portion with a polythene
                     tape to keep the union intact.
                 •   After the graft is completed, cut the stock above
                     the union.
                                       Activity 2
               Demonstrate cleft grafting.
               Material required: Rootstock, scion, grafting knife,
               grafting tape, etc.
               Selection of material
                  • The scion must be taken from the terminal shoot of
                     current season growth with 3–5 buds.
78
                                                              GARDENER – CLASS XI
        •    The scion shoot is defoliated about two weeks ahead           NOTES
             of separation from the mother plant.
  Procedure
    • Raise the rootstock of the required plant in a
       poly bag.
    • Select 4 to 5-month old suitable rootstock and cut
       the terminal portion (head back).
    • A sharp vertical straight downward cut of 3–5 cm is
       given at the centre of the stem.
    • Two slanting cuts of the same length (3−5 cm), as
       in the rootstock, are given on the scion shoot at the
       opposite side towards the base.
    • Insert the scion in a way that it matches the
       cambium layer at least on one side with the stock.
    • Tie the grafted portion firmly with a polythene tape.
    • After successful union, the terminal buds of the
       scion begin to sprout.
    • Loosen or remove the polythene tape to allow the
       shoot to grow normally.
    • Stake the newly grafted plant.
                                                                                   79
PLANT PROPAGATION
     NOTES     3. Rootstock and scion are required in                       .
                  (a) layering              (b) grafting
                  (c) cutting               (d) gootee
               4. Epicotyl grafting is also called
                  grafting.
                  (a) stone                 (b) cleft
                  (c) wedge                 (d) whip grafting
               5. Top working can be done by                            .
                  (a) top grafting          (b) stone grafting
                  (c) veneer grafting       (d) tongue grafting
C. Subjective Questions
A B
             Types of budding
             There are many methods or techniques of inserting
             bud into the rootstock. Some of the common methods
             are as follows.
80
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                                                             GARDENER – CLASS XI
T – budding
Since a ‘T’-shaped incision is made for bud insertion
on the rootstock, it is called T – budding. T – budding
is also called ‘shield budding’ as the bud used for
insertion is in the shape of a ‘shield’. It is widely used
for propagating fruit trees and ornamental plants. In
this method, the rootstocks of compatible plants are
raised in beds or poly bags.
    One-year old rootstock of a healthy and vigorous
growth is selected. A T-shaped cut is made at a height
of 15−25 cm from the ground level on the rootstock
with the help of a sharp knife. Also, a vertical cut,
extending up to 2.5−3.75 cm is given on the bark in
the internodes. At the top of this vertical cut, another
horizontal cut is given in such a way that the two
cuts resemble the shape of ‘T’. Now, the bark of the
cut portion is loosened. The bud from the desirable
plant is selected. The shield-shaped bud along with
the woody chip is removed from the scion stick. The
bud is inserted in the T-shaped cut in the stock. After
the insertion of the bud with the help of a budding
knife, the bud and stock are firmly wrapped with a
polyethylene strip, exposing the bud. The bud sprouts
within a month. Examples are rose, apple, pear,
peach, apricot, cherry, sweet orange, etc.
                                                                                                         81
                                                                                                         8
PLANT PROPAGATION
                                  Patch budding
                                  A rectangular patch of bark, measuring 2.41.5 cm
                                  (length and width), is completely removed from the
                                  internodes of the stock plant. A similar patch of bark
                                  with a healthy bud is removed from the scion bud
                                  stick. This patch is placed on the cut portion of the
                                  stock and wrapped with a polyethylene strip, keeping
                                  the bud exposed. This type of budding is useful for the
                                  propagation of plants having a thick bark. Examples
                                  are amla, mango, jamun, rubber, etc.
(a) Scion   (b) Patch of   (c) Patch of bark to       (d) Patch      (e) Patch bud      (f) Patch
             bark with                                                                              (g) Sprouted
                               be removed             of bark          fitted with         bud
                bud          from the stock                                             wrapped      bud scion
                                                      removed          the stock
                                  Ring budding
                                  In this method, a bark of approximately 3−6 cm wide
                                  in ring form is removed from the stock. The same
                                  dimension of bark with a healthy bud is removed from
                                  the scion bud stick and placed on the stalk. After placing
                                  the ring in position, tie it with a polythene strip, keeping
                                                                      the bud exposed, e.g.,
                                                                      ber and cherry.
                                                                                     Flute budding
                                                                                     This is a slight
                                                                                     modification of ring
                                                                                     budding. Instead of
                                                                                     removing the complete
    (a)           (b)          (c)                    (d)              (e)           ring, a narrow portion
Rootstock   Ring-shaped Insertion of              Wrapping        Sprouting          of the bark about 1/8 of
preparation      bud        the bud               of the bud      of the bud
                                                                                     its circumference is left
                     Fig. 3.19 (a–e): Ring                                           on the stock. A similar
                     budding
 82
                                                                                             GARDENER – CLASS XI
    portion of the scion
    is removed along
    with the bud and
    is fitted on the cut
    portion of the stock.
    The bark of the stock
    and bud are tied with
    a polyethylene strip,
    exposing the growing
    point e.g., ber.
    Forkert budding            (a) Rootstock                      (c) Insertion and   (d) Completed
                                  cut to fit   (b) Scion bud         wrapping of        bud graft
    In forkert method,            bud ring                             the bud
    a horizontal cut at
the internodes of the                            Fig. 3.20 (a–d): Flute budding
    selected rootstock is
    given at a distance of 20−25 cm above the ground level.
    Two vertical cuts from either ends of the horizontal cut,
    extending downwards, are taken and a flap of the bark
    is pulled out, exposing a rectangular woody portion of
    about 2.55 cm on the rootstock. A rectangular piece
    of bark of the same size along with a matured bud is
    removed from the bud stick with the help of a budding
    knife. This piece of bark is then shifted over the exposed
                                                                                                83
    PLANT PROPAGATION
                           portion on the rootstock. The flap of the bark is loosened
                           and tied to its original position, covering the scion bud
                           fitted inside. After three weeks, the polyethylene strip
                           is removed and the flap of the bark is pulled out for
                           observation. If the bud shows sprouting signs, the flap
                           is removed by giving a horizontal cut on the downside.
                           Then, the polyethylene strip is wrapped, keeping the
                           growing point exposed. The bud sprouts within 3 – 5
                           weeks of budding operation. Examples are cashew nut,
                           jackfruit, mango, etc.
                                Chip budding
                                This method is followed when the bark is thin and cannot
                                be removed easily. In this method, a piece of thin bark,
                                                                  along    with     some
                                                                  wood       piece,    is
                                                                  removed       between
                                                                  two nodes of the
                                                                  rootstock, and the
                                                                  same size of chip,
                                                                  which is similar in
                                                                  shape       and      is
                                                                  collected from the
(a) Operated   (b) Insertion     (c) Wrapped     (d) Emerging
    stock       of the bud           bud              bud
                                                                  scion, is placed on
                                                                  the rootstock. This
                                                                  is mostly practised
                     Fig. 3.22 (a–d): Chip budding
                                                                  in February−March.
                                Fruits like apple, grapes and pear can be propagated
                                through this technique.
                           Tissue culture
                           It is a technique for growing plant tissues isolated from
                           the parent plant in an artificial medium and controlled
                           environment over a prolonged period under aseptic
                           conditions. It is used on commercial scale in gerbera,
                           orchid, banana, carnation, anthurium, etc. It is based on
                           the phenomenon of ‘totipotency’ of a cell, which denotes
                           the capacity of a plant cell to regenerate into a full-
                           fledged plant having different organs.
                               Callus is produced on explant in vitro due to
                           wounding and growth substances, either endogenous
                           or supplied exogenous in the medium. For the collection
 84
                                                                       GARDENER – CLASS XI
                    of explants, plant parts, such as stem, root or leaves
                                                                      83
PLANT PROPAGATION
can be used. After disinfestation, they are induced to        NOTES
form ‘callus’. Examples are banana, papaya, gerbera,
carnation, rose, orchid, etc.
Bulb
Bulb is a specialised underground structure having
a flat basal stem and surrounded by fleshy scales,
e.g., onion, tuberose, amaryllis. Structurally, bulbs
are tunicated and non-tunicated. In tunicated
bulbs, the outer layer of scales is converted into dry
membranous covering, which gives protection, e.g.,
onion, tuberose, amaryllus, tulip, etc. Non-tunicated
bulbs do not possess the enveloping dry covering and
are represented by lilium.
Corm
Corm is an underground modified solid or compressed
stem oriented vertically in the side having nodes and
buds, e.g., gladiolus, crocus, etc.
Tuber
It is an underground storage organ having special
swollen modified stem or roots, e.g., root tuber like
dahlia, caladium, dioscorea, Jerusalem artichoke, etc;
and stem tuber like begonia, potato, etc.
Rhizome
A modified stem of some plant growing horizontally
just below the ground surface, e.g., canna, ferns, ginger,
iris, etc.
Runner
It is a modified stalk, which is creeping in nature,
produced in the leaf axil and grows out from the parent
plant. It grows horizontally along the ground, where
roots are produced at the nodes, which can be used
                                                                           85
PLANT
  86 PROPAGATION
  84
                                                             GARDENER – CLASS XI
     (a) Runner (chlorophytum)                         (b) Sucker                 (c) Rhizome (canna)
(d) Corm (gladiolus) (e) Bulb (tuberose) (f) Root tuber (dahlia)
86
                                                                                      GARDENER – CLASS XI
                    Practical Exercise                               NOTES
                              Activity
  Demonstrate T– budding.
  Material required: Secateurs, budding knife, rose rootstock,
  scion and budding tape
  Procedure
     • Select and prepare a rootstock with the help of
        secateurs.
     • Make a ‘T’ shape cut with the help of a budding knife.
     • Gently open the bark of the rootstock with the help
        of a bud opener.
     • Prepare the bud with the help of a budding knife.
     • Insert a shield bud into the ‘T’ cut.
     • Wrap this portion with a polythene strip.
                                                                             87
PLANT PROPAGATION
     NOTES     5.     Multiplication of a plant in controlled environment
                      under aseptic condition is known as                    .
                      (a) tissue culture               (b) protected culture
                      (c) hydroponic                   (d) soilless culture
             C. Subjective Questions
               1. What is budding?
               2. Describe the procedure of T-budding
A B
88
                                                                  GARDENER – CLASS XI