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Liquid Reagent Kinetic Determination. SFBC Store at 2-8 ºC: The Following Symbols Are Used On Labels

This document outlines the kinetic determination of lactate dehydrogenase (LDH) according to SFBC recommendations, detailing the reaction process, calculation method, and reference values for different temperatures. It specifies sample requirements, reagent concentrations, and preparation instructions, as well as potential interferences and safety notes. The document also includes procedural guidelines for conducting the assay and emphasizes the importance of each laboratory establishing its own normal range.

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Zenebe Abebe
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58 views1 page

Liquid Reagent Kinetic Determination. SFBC Store at 2-8 ºC: The Following Symbols Are Used On Labels

This document outlines the kinetic determination of lactate dehydrogenase (LDH) according to SFBC recommendations, detailing the reaction process, calculation method, and reference values for different temperatures. It specifies sample requirements, reagent concentrations, and preparation instructions, as well as potential interferences and safety notes. The document also includes procedural guidelines for conducting the assay and emphasizes the importance of each laboratory establishing its own normal range.

Uploaded by

Zenebe Abebe
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Liquid Reagent

LDH
Kinetic determination. SFBC
Store at 2-8 ºC

PRINCIPLE

Kinetic determination of the lactate dehydrogenase CALCULATION


according to the SFBC recommendations by the U/L = Δ O.D/min x F
following reaction: 340 nm F = 8095

Pyruvate + NADH + H+ LDH


LINEARITY
L-Lactate + NAD+ If there is not any kinetic or if Δ O.D/min. exceeds
0.1 at 334-340 nm at 25ºC, or if Δ OD/min. exceeds
REFERENCE VALUES 0.05 at 366 nm, repeat test using serum diluted 1/5
or 1/10 with physiological saline.
25ºC 30º C 37º C
Adult < 240 U/l < 330 U/l < 480 U/l SPECIFICATION
Children < 500 < 690 < 1000
Bilirubin 0.15g/l, lipid 10g/l, glucose 3g/l and
It is recommended that each laboratory should ascorbic acid 0.45g/l do not interfere with the assay
assign its own normal range. up to the given levels

SAMPLES NOTES
Solution 1 contains sodium azide, avoid ingestion or
Serum free of hemolysis or heparinized or EDTA contact with skin.
plasma. LDH in sample is stable for 1 day at 2-8ºC.
PRESENTATION
REAGENTS
50 ml Cat No 2401 60 Tests
180 ml Cat No 2402 180 Tests
Concentration in the test A+B :
Tris buffer 50 mmol/l
BIBLIOGRAPHY
NADH 0.18 mmol/l
Z. Klin. Chem. U. Klin. Biochem, 1970, 8,658,
Pyruvate 0.6 mmol/l
1972,10, 182.
PREPARATION OF WORKING REAGENT
Reagent A and B are ready to use. If a monoreagent
The following symbols are used on labels
procedure is preferred then the reagent must be
mixed in the ration 4 parts of A to 1 part of B. The IVD For in vitro diagnostic use
working reagent is stable for one month at 2-8ºC
Use day (last day of the month)
PROCEDURE

Wavelength 340 nm Temperature limitation


Temperature 25ºC/30ºC/37º C
Zero adjustment Air or distilled water LOT Batch code
Cuvette 1 cm light path
Method Kinetic - decreasing
REF Code
If the absorbance of the working reagent is lower
than 1.0 at 334 nm the reagent can not be used.
Sample 20 µL
Working reagent 1.0 ml
Mix and after a 1 minute incubation, measure the
change of optical density per minute
(Δ O.D/min.) for 3 minutes.

04/2014

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