DIPLOMA IN PHARMACY – 2nd YEAR
LESSON PLAN
BIOCHEMISTRY & CLINICAL PATHOLOGY – THEORY
Course Code: ER20-23T 75 Hours (3 Hours/week)
Name of Tutor/Teacher: Sh. Mohd. Hamid, Guest Faculty in Pharmacy
Sh. Dalbir Singh, Senior Lecturer in Pharmacy (Tutorial)
Schedule of Classes: Theory: Monday: 12.00 – 01.00 PM, Monday: 02.00 – 03.00 PM,
Wednesday: 02.00 – 03.00 PM, Wednesday: 03.00 – 04.00 PM (Tutorial)
Scope: This course is designed to impart basic knowledge on the study of structure and
functions of biomolecules and the chemical processes associated with living cells in normal
and abnormal states. The course also emphasizes on the clinical pathology of blood and urine.
Course Objectives: This course will discuss the following at the fundamental level
1. Structure and functions of biomolecules.
2. Catalytic activity, diagnostic and therapeutic importance of enzymes.
3. Metabolic pathways of biomolecules in health and illness (metabolic disorders).
4. Biochemical principles of organ function tests and their clinical significance.
5. Qualitative and quantitative determination of biomolecules/metabolites in the
biological sample.
6. Clinical pathology of blood and urine.
Course Outcomes: Upon successful completion of this course, the students will beable
to
CO2.3T.1: Describe the functions of biomolecules
CO2.3T.2: Discuss the various functions of enzymes in the human system
CO2.3T.3: Explain the metabolic pathways of biomolecules in both physiological and
pathological conditions
CO2.3T.4: Describe the principles of organ function tests and their clinical significances
CO2.3T.5: Determine the biomolecules/metabolites in the given biological samples,
both qualitatively and quantitatively
CO2.3T.6: Describe the clinical pathology of blood and urine
1 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Reason for Taught Verified
Chapter Topic Date Hour CO PO Coverage compensation
discrepancy by by
in backlog
1 Scope of biochemistry in Pharmacy 1
Introduction
to Cell and its biochemical organization 2
Biochemistry
Definition, classification with examples, chemical
1
properties
Monosaccharides - Structure of glucose, fructose,
2
2 and galactose
Carbohydrates Disaccharides - structure of maltose, lactose, and
3
sucrose
Polysaccharides - chemical nature of starch and
4
glycogen
Qualitative tests and biological role of carbohydrates 5
Definition, classification of proteins based on
1
composition and solubility with examples
Definition, classification of amino acids based on
chemical nature and nutritional requirements with 2
3 examples
Proteins Structure of proteins (four levels of organization of
3
protein structure)
Qualitative tests and biological role of proteins and
4
amino acids
Diseases related to malnutrition of proteins 5
Definition, classification with examples 1
Structure and properties of triglycerides (oils and
2
fats)
Fatty acid classification – Based on chemical and
3
4 nutritional requirements with examples
Lipids Structure and functions of cholesterol in the body 4
Lipoproteins - types, composition and functions in
5
the body
Qualitative tests and functions of lipids 6
2 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Reason for Taught Verified
Chapter Topic Date Hour CO PO Coverage compensation
discrepancy by by
in backlog
Definition, purine and pyrimidine bases 1
Components of nucleosides and nucleotides with
2
5 examples
Nucleic acids Structure of DNA (Watson and Crick model) and
3
functions
Structure of RNA and functions 4
Definition, properties and IUB and MB classification 1
Factors affecting enzyme activity 2
Mechanism of action of enzymes, Enzyme inhibitors-
6 3
1
Enzymes
Mechanism of action of enzymes, Enzyme inhibitors-
4
2
Therapeutic and pharmaceutical importance of
5
enzymes
1
Definition and classification with examples
2
7 3
Vitamins Sources, chemical nature, functions, coenzyme form,
4
recommended dietary requirements, deficiency
5
diseases of fat-and water-soluble vitamins
6
Metabolism of Carbohydrates: Glycolysis 1
TCA cycle 2
8
Metabolism Glycogen metabolism 3
(Study of
cycle/pathways Regulation of blood glucose level 4
without
chemical Diseases related to abnormal metabolism of 5
structures)
Carbohydrates 6
Metabolism of lipids: Lipolysis 7
3 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Reason for Taught Verified
Chapter Topic Date Hour CO PO Coverage compensation
discrepancy by by
in backlog
β-oxidation of Fatty acid (Palmitic acid) 8
Ketogenesis and ketolysis 9
Diseases related to abnormal metabolism of lipids
10
such as Ketoacidosis
Fatty liver, Hypercholesterolemia 11
Metabolism of Amino acids (Proteins): General
8 reactions of amino acids and its significance- 12
Metabolism Transamination
(Study of
cycle/pathways Deamination 13
without
chemical Urea cycle and decarboxylation 14
structures)
Diseases related to abnormal metabolism of amino 15
acids
16
Disorders of ammonia metabolism, phenylketonuria,
17
alkaptonuria and Jaundice.
18
Biological oxidation: Electron transport chain
19
and
Oxidative phosphorylation
20
Minerals: Types,
1
Functions,
2
9
Deficiency diseases
Minerals 3
4
recommended dietary requirements
5
4 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Reason for Taught Verified
Chapter Topic Date Hour CO PO Coverage compensation
discrepancy by by
in backlog
Distribution, functions of water in the body
1
Water turnover and balance
2
10 Electrolyte composition of the body fluids, Dietary
Water and 3
intake of electrolyte and Electrolyte balance
Electrolytes
Dehydration, causes of dehydration and oral
4
rehydration therapy
5
Introduction to Biotechnology
11 1
Functions of kidney and routinely performed tests to
1
assess the functions of kidney and their clinical
significances 2
12 Functions of liver and routinely performed tests to
3
assess the functions of liver and their clinical
Organ function significances 4
tests
Lipid profile tests and its clinical significances
5
6
Lymphocytes and Platelets, their role in health and
1
disease
12 2
Erythrocytes - Abnormal cells and their significance 3
Introduction
to Pathology of 4
Blood and
Normal and Abnormal constituents of Urine and
Urine 5
their significance
6
5 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
BIOCHEMISTRY & CLINICAL PATHOLOGY – PRACTICAL
Course Code: ER20-23P 50 Hours (2 Hours/week/Batch)
Name of Tutor/Teacher: Sh. Dalbir Singh, Senior Lecturer in Pharmacy (Tutorial)
Schedule of Classes: Practical: Monday: 09.00 – 11.00 AM, Wednesday: 09.00 – 11.00 AM,
Tuesday: 09.00 – 11.00 AM
Scope: This course is designed to train the students in the qualitative testing of various
biomolecules and testing of biological samples for determination of normal and abnormal
constituents.
Course Objectives: This course will train and provide hands-on experiences on thefollowing
1. Qualitative determination of biomolecules/metabolites in simulated biological samples.
2. Determination of normal and abnormal constituents of simulated blood and urine
samples.
Course Outcomes: Upon successful completion of this course, the students will be able to
CO2.3P.1: Qualitatively determine the biomolecules/metabolites in the given biological
samples.
CO2.3P.2: Determine the normal and abnormal constituents in blood and urine samples and
interpret the results of such testing.
CO2.3P.3: Study the various Biochemical reactions.
6 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Exp. Reason for
Experiment Batch Date CO PO Coverage compensation Taught by Verified by
No. discrepancy
in backlog
Qualitative analysis of carbohydrates-1 A
1 B
C
A
2 Qualitative analysis of carbohydrates-2 B
C
A
3 Qualitative analysis of carbohydrates-3 B
C
A
4 Qualitative analysis of carbohydrates-4
B
C
A
Qualitative analysis of Proteins and amino acids-1
5 B
C
A
6 Qualitative analysis of Proteins and amino acids-2 B
C
A
7 Qualitative analysis of Proteins and amino acids-3 B
C
A
8 Qualitative analysis of Proteins and amino acids-4 B
C
A
9 Qualitative analysis of lipids-1 B
C
A
10 Qualitative analysis of lipids-2 B
C
7 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Exp. Reason for
Experiment Batch Date CO PO Coverage compensation Taught by Verified by
No. discrepancy
in backlog
A
Qualitative analysis of urine for normal and abnormal
11 B
constituents-1
C
A
Qualitative analysis of urine for normal and abnormal
12 B
constituents – 2
C
Qualitative analysis of urine for normal and abnormal A
13 B
constituents-
C
A
Qualitative analysis of urine for normal and abnormal
14 B
constituents-4
C
Determination of constituents of urine (glucose, creatinine, A
15 chlorides)-1 B
C
Determination of constituents of urine (glucose, creatinine, A
16 chlorides)-2 B
C
Determination of constituents of urine (glucose, creatinine, 3A
17 chlorides)-3 B
C
Determination of constituents of blood/serum A
18 (simulated) (Creatine, glucose,cholesterol, Calcium, Urea, B
SGOT/SGPT)-1 C
Determination of constituents of blood/serum A
19 (simulated) (Creatine, glucose,cholesterol, Calcium, Urea, B
SGOT/SGPT)-2 C
Determination of constituents of blood/serum A
20 (simulated) (Creatine, glucose,cholesterol, Calcium, Urea, B
SGOT/SGPT)-3 C
Determination of constituents of blood/serum A
21 (simulated) (Creatine, glucose,cholesterol, Calcium, Urea, B
SGOT/SGPT)-4 C
Determination of constituents of blood/serum A
22 (simulated) (Creatine, glucose,cholesterol, Calcium, Urea, B
SGOT/SGPT)-5 C
8 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Plans for
Exp. Reason for
Experiment Batch Date CO PO Coverage compensation Taught by Verified by
No. discrepancy
in backlog
Determination of constituents of blood/serum A
23 (simulated) (Creatine, glucose, cholesterol, Calcium, Urea, B
SGOT/SGPT) C
A
Study the hydrolysis of starch from acid and salivary
24 B
amylase enzyme-1
C
A
Study the hydrolysis of starch from acid and salivary
25 B
amylase enzyme-2
C
9 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
Assignments
The students shall be asked to submit written assignments on Various Pathology Lab Reports
(One assignment per student per sessional period. i.e., a minimum of THREE assignments per
student).
Recommended Books (Latest Edition)
1. Essentials of Biochemistry by U. Satyanarayana, Books and Allied (P) Ltd.
2. A Textbook of Biochemistry by A.V.S.S. Rama Rao, UBS Publishers’ Distributors Pvt. Ltd.
3. Practical Biochemistry by R.C. Gupta and S. Bhargava.
4. Laboratory manual of Biochemistry by Pattabiraman and Sitaram Acharya
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10 | Lesson Plan: Biochemistry & Clinical Pathology D. Pharm. (2nd Year)
