Kanachur Institute of Medical Sciences, Natekal

Department of Biochemistry
I MBBS (2023-2024)- I Internal Assessment Examinati
Date: 06.01.2024 on
Time: 9 AM- 12 PM
Max Marks: 80
Duration: 3 hours
1. Write your Name and Register number on the
answer booklet.
2. Write neatly and legibly.
3. Draw neat diagrams wherever necessary.
4. Any malpractice will be severely dealt with.

I. LONG ESSAYS:
VA 15 year old boy was admitted with complaints of fatigu 2x10=20 marks
e, recurrent fever and pain
in arms and legs. His peripheral blood smear show
ed sickled emhrofy1es and a
hemoglobin concentration of 6 gm%.
a) Name the molecular defect in this disorder. (1)
b) Explain the mechanism of sickling. (2)
c) Reason out why these patients show resistance to
malaria. (2)
M Explain the steps of heme synthesis. Add a note on its
regulation. (3+2) ...-
¥Wh at is the normal blood level of calcium? Write
four functions of calcium. What are
the mechanisms by which calcium homeostasis is main
tained. (1 +4+5)v
II. SHORT ESSAYS:
(_,.,-/A chronic alcoholic patient presented with skin 7x5=35 marks
lesions on exposed areas of the body.
He also complained of frequent episodes of passi
ng loose stools and memory
impairment. A provisional diagnosis of pellagra was
made.
a) Nam e the vitamin deficiency causing pellagra. (1)
"'-\
b) Mention its coenzyme forms. (1)
;:,) Write three reactions which require them as coenz
yme. (3) .
~ 9pla in the fluid mosaic model of plasm
a membrane with labeled diagram. (3+2)
¥.Di scus s the effect of substrate concentration and
temperature on enzyme catalysed
ctions. (2.5+2.5)
assify enzymes. Give one reaction for each class. (1 +4)
fine porphyria. Name the enzyme defect in different
types of porphyria. Explain
4~h nica l features of acute intermittent porphyria.
(1 + 3+1)
v8'. 1)efi ne and describe Allosteric regulation of enzym es. Give one example each for
/ Allosteric activator and inhibitor. (1+2+1+1)
, , ¥.Na me five homo-polysaccharides. Write their
composition and biological importance.
~ (2+2+1)

IJI. SHORT ANSWERS:

1.eJOefine anaplerosis. Describe any two anapler~tic 5x3=15 marks
reactions of citric acid cycle. (1 +2)
---~.,."~hat is substrate level phosphorylation? Give two
examples. (1 +2)
'" ~~r ite the composition and functions of collagen.
(2+ 1)
Which vitamin deficiency is likely to develop in patie
nts on Isoniazid, an anti tuber Bl-,
cular drug? What is the coenzyme form of this vitam
;>:action dependent on this nutrient. (1 + 1+ 1)
in? Write one biochemical ~
)4. What is the end product of aerobic glycolysis? Illust
rate the fate of this compound.
(1+2)
 IV. MULTIPLE CHOICE QUESTIONS:
5x1=5 marks
15 • i) Carbon dioxide is carried in blood
in all the forms, except:
A) Dissolved form Hffsohydric transport
C) Carboxyhemoglobin D) Carbaminohemoglobin
15. ii) Which enzyme is not involved in
catabolism of heme?
A) Heme oxygenase B) UDP glucuronyl transferase
,- "\ orru"liverdin reductase D) Ferrochelatase
~.i ii) An example for cardiac glycosid
e is --------
A) Digitalis B) Sorbitol
C) Glucuronic acid D(Mannosamine
15. iv) 0steogenesis imperfecta is due to
defect in
A) E)s tin B) Keratin
O),.-,Collagen D) Heparin
15. v) GLUT-4 is the major glucose tran
sporter in
\JX')Liver B) Pancreas
C) Adipose tissue D) Erythrocytes
16. i) Enzymes which are synthesized in 5x1=5 marks
inactive forms are called --------
A) Coenzymes B) Apq,enzymes
::-7 C) Lysozymes ~e nzy me s
16.) i) In Wiison's disease copper accumul
ates in all of the following tissues except
'-- -' A) Eyes
.-13) Spleen
C) Brain D) Liver
16. iij/W hich of the following derivative
/ 1ntr
• aerama• 1pressure ? of monosaccharide is used to reduce raise
d
.
_,, -~~t~fI
16. iv) Wh
~ ~~ ::o l
ich one of the following enzymes is present
'-' ~X i t< ~~
in lysosomes? ~~ ~-
~ho sph olip ase B) Aldolase •
C) Acetyl CoA carboxylase D) HMG CoA reduc!ase
16. v) The vitamin that acts as a coenzym
~r~anske.tolas.e:'activity is--------
A) Pyridoxine '""~Thiamine
C) Biotin D) Folate
I Kanachur Institute of Me
Department of Biochemist
dical Sciences, Natekal
ry . .
at1on
•
ernal Assessment Exam 1n
I MBBS (2023-2024)- II Int Tim t· AM
e: 9 - 12 PM
. 3 hours
D
ura 100 .
Date: 28.03.2024
M ax M ark s: 80 booklet.
e yo ur Na me an d Re gis ter number on the answer
1. Writ
y.
2. Write neatly and legibl
gra ms wh erever ne ce ssa ~.
3. Draw neat dia
be severely dealt with.
4. Any malpractice will
. 2x.10=20 mark.s
. ous ga stn c
I. LONG ESSAYS: era ted for int est ina l ob struction and. had continu
I. A 50 ear old man was
op sur ge on
oratory findings wh en the
• ~tion 1or e fol low ing are the lab
aspira c 3 days. Th
e
came for the review. Value Biological Reference Ra ng
Pa tie nt
Investigation 7.35 - 7.45
7.5 5
Blood pH
ol/ L 20-30 mm ol/ L
on ate 45 mm
Plasma Bicarb 35-45 mmol/1
60 mmol/L
pCO2 136 - 145 Me q/L
Na+ 140 Meq/L
3.5 - 5 Me q// L
K+ 2.5 Meq/L
order 1n above case. (1)
a) Name the acid base dis
chanism. (3)
b) Write compensatory me
n of acid base balance. (4)
c) Describe renal regulatio ere nce interval. (2) . . .
gap ? Me nti on its ref ort of ids.
lip
d) What is anion fer ent lip op roteins involved 1n transp
nti on the dif
2. Define lipoproteins. Me lipids under the fol low ing
heading.
in me tab oli sm of die tar y
Expla
ids
a) Assembly of dietary lip
_
bL ~s po rt_ __ _ _
l tissues
c) Utilization in periphera
+2+2+2+2)
d) Metabolic fate. (1 + 1
7x5=35 marks
II. SHOR T ES SA YS : ~ . He was obese
sen ted wi th exc ruc iating pain in the great toe
(
3. A 45 year old ma le pre um uric acid
of con sum pti on of alc oh ol. On investigation his ser
and had long history
level was 12.1 mg/dl. (1)
acid level in the above case.
a) Interpret the serum uric
uricemia. (2)
b) Write four causes of hyper in treatment of Gout. (2)
bio che mi cal basis of giving allopurinol at are
c) Ex pla in the
on the ir nu trit ion al sig nif icance giving examples. Wh
ed
4. Classify aminoacids bas Give one exan1ple. (3+ 1+ 1)
non pro tei n am ino aci ds. rylation.
ory and illu str ate the pro cess of oxidative phospho
5. State chemiosmotic the ficiency
bio che mi cal fun cti on s, requiren1ent an d de
6. Describe sources, am two
B 12. (1 + 1+ 1+2)
manifestations of vit in an d muscle
lys is. Wr ite the dif fer ences between liv er
7. Explain glycog(4+ eno
glycogenolysis. 1)
.
8. Describe the steps inv olved in heme degradation
th ketonuria.
og ene sis . Me nti on tw o conditions associated wi
9. Describe steps in ket
(3+2)
 III. SHORT ANSWERS: 5x3=15 marks
10. What is detoxification? Explain with two examples the process of detoxification by
conjugation mechanism. (1 +2)
11. Explain the biochemical role of vitamin E. What is its inter-relati~nship with
selenium. (1 +2)
12. Enumerate three conditions causing appearances of reducing sugars in urine.
13. Write the serum and urinary biochemical findings in obstructive jaundice. Write the
type of Van den Bergh reaction seen in above case. (2+1)
14. Name three phospholipids and write their biological importance.

IV. MULTIPLE CHOICE QUESTIONS: 5xl=5 marks

··-
15. i) Which of the following is a semi essential amino acid?
A) Arginine B) Alanine
C) Aspartic acid D) Glycine
15. ii) Nitrogenous base present both in DNA and RNA is
A) Uracil B) Thymine
.C) Cytosine D) Xanthosine
15. iii) the protein part present in nucleoprotein is --------
A) Globin B) Opsin
#C) Histones D) Apoferritin
15. iv) ----------- is known to act as a phY.siological uncoupler
A) Valinomysin BJThyroxine
C) Carboxine D) 0 ligomycin
15. v) Phospholipid deficiency in Respiratory Distress Syndrome is
A) Sphingomyelin B) Phosphatidyl ethanolamine
C) Plasmalogen ~ D) Lecithin
5x1=5 marks
16. i) Heme is converted to bilirubin in -
A) Mitochondria ..,Bj Microsomes
C) Golgi apparatus D) Plasma membrane
16. ii) The amino acid which do not allow formation of alpha-helix is
A) Glutamate B)' Proline
C) Tyrosine D) Histidine
16. iii) Which coenzyme is responsible for carboxylation reactions
A) Biotin B) FAD
C)NAD D) TPP
16. iv) Hormone insulin activates which of the following pathway
A) Glycogenolysis B) Gluconeogenesis
C) Glycolysis D) Lipolysis
16. v) N3 of purine ring is donated by
A) Aspartate B) Glycine
.¢ Glutamine D) Ammonia
I -,

Kanac hur Institu te of Medic al Sciences, Natek al

Depar tment of Bioch ernistr y
I l\1BBS (2023- 2024)- Ill Intern al Asses sment Exam inatio n - Paper 1
Date: 20.06.2024 Tilne: 9 AM- 12 PM
Max l\1ark s: 100 Durat ion: 3 hours
bookle t.
1. Write your Nan1e and Regist er numb er on the answe r
2. Write neatly and legibly .
3. Draw neat diagra111s where ver necess ary.
4. Any nrnlpracticc will be severe ly dealt with.

2x10= 20 marks
I. LONG ESSA VS:
History revealed that
1. A 25 year old patient was brought to casualty in comatose state.
n1ical analys is of blood and urine showed
he was not taking insulin regularly. Bioche
the following findings.
Blood sugar- 510 mg/di
U1ine for Rothera's test and Benedict's _test positive.
e value. (2)
a) Suggest the probable diagnosis and interpret the plasma glucos
ed hemog lobin (HbAIC) 111 the
b) Explain the rationale behind the use of glycat
HbA IC? (2+ 1)
above diagnosis. What is the reference range for
c) Name the con1pounds which give Rothera's test positive.
(1)
ketone bodies. (4)
d) Explain the steps in the synthesis and breakdown of
n the energetics of the
2. Write the steps in beta-oxidation of palmitic acid. Explai
e?(6+3 +1)
pathway. What is the biochen1ical defect in Refsum diseas

II. SHOR T ESSA YS: 8X5=4 0 marks

pment of spongy and
3. A 40 year old navy officer contacted his physician for develo
and complained of
bleeding gums. He also had loosening of teeth, pain in the joints
rine tour for 40 days and
,. poor wound healing. History revealed that he was in subma
was only on staple diet.
a) Name the vitan1in deficiency and disorder. (1)
b) Give RDA, two sources and two functions of above vitamin.
(3)
basis of spongy and bleedi ng gums. (1)
c) Explain the biochemical
examples of dietary fibers.
J\ (' 4. What arc dietary fibers? Write their functions. Give four
(1+2+ 2)
.c" r (1+2+ 1+1)
V' 5. Explain the sources, absorption, transport and storage of iron.
ing in the liver and adipos e tissue in well-fed
v,. ((5. Explain the metabolic adaptations occurr
state.
v'- 7. a) Define basal metabolic rate (BMR). Enumerate
four factors affecting BMR. (1+2)
b) Compare and contrast between kwashiorkor and n1arasrnus. (2)
les. (3+2)
• ~ • 8. Explain compel itivc inhibition of enzyme with two examp
hypothesis with a neat
9. Define oxidative phosphorylation. Explain chemiosmotic
n transport chain.
labeled diagram. Name two site specific inhibitors of electro
(1+3+ 1)
tory acidosis. Write the
~O. Define respiratory acidosis. Enumerate the causes for respira
_ \) compensatory mechanism in respiratory acidosis. ( l +2+
2)

I0X3= 30 marks

~ V
,, lll. SIIOR T ANSW ERS:
D. Wh_at is So1:bitol pathway? Write the significance of Sorbit
ol pathway. (2+ I) (
~nzyrnc.
r 12. Writ~ the diagnostic significance or 1.:ach of the rollowing serum
•

, (a) Lipase (b) /\lanin e transaminase (c) CKMB

\ 13. Name any two proteins of extrac ellular matrix . Write the post- transli-ltional
mod di cation of collag en and it's import ance. ( I +2)

l I
 l4. What ar
tl1 1. ~ ' c mucopolysaccharides? Name any two mucopolysaccharides and write
e r mnctions. (I +2)
15
j_Wh~t _is fatty l~ver? Enumerate two causes of fatty liver. Explain the role of'
f;)ipot~ op1c factors m preventing fatty liver. (1 + 1+I)
lo/ Wnte the normal biological reterencc value for serum sodium. Mention any two
\ causes of hypernatrernia. (l +2) ,
@What is fluorosis? \1/rite the clinical features associated with skeletal and dental
fluorosis. (1 +2)
CJ 18. What is lepti1f? Mention it's biological importance. Cl +2)
19. Explain fluid mosaic model of cell membrane with a neat labeled diagram. (2+ I)
20. \1/hat is the role of antidiuretic hormone (ADH) in water balance? Name the disorder
associated with this deficiency. (2+ l)

IV. l\1UL TIPLE CHOICE QUESTIONS: 10xl=10 marks

2 I., i) The marker enzyme for microsomes is
t A) ATP synthase B) Cathepsin
' £) Glucose-6-p hosphatase P1 Lactate dehydrogenase
21.i'ii)The vitamin that acts as a coenzyrne for transketolase activity is
\ A) Pyridoxine ff)Thiamine
"' C) Biotin D) Folate
21. iii) Renal theshold for glucose is
Ji'() / A) 90 mg/<ll 13) 120 mg/di
/ C) 150 mg/dl Jd? 180 mg/di
21. iv) Which of the following enzyme is specific to gluconeogenesis?
\ , A) Phosphohexose isomerase "Qfl>yruvate carboxylase
/ C) Aldolase D) Enolase
21. v) Phospholipid deficiency in respiratory distress syndrome is
/A) Sphingomyelin F3) Phosphatidyl ethanolamine
' \ C) Plasmalogen B;'tecithin
22. i) The vitamin deficiency that is likely to develop in patients on Isoniazid, an
antitubercular dn1g is
)\" A) Niacin B) Thi~min_e
C) Riboflavin B)Pyndoxme
fi. ii) \1/hich of the following hormones DOJ;S NOT have any effect on ECF volume?
, A) Antidiuretic hormone 8) Aluosterone
~ er Calcitriol D) Ren in ✓
2). iii) Positive nitrogen balance is seen in
\ / ~ Pregnancy 8) Malig~a~cies
.. C) Malnutrition D) Chrome illness
22. iv) Bile acid is derived from
1 / 4 Cholesterol B) Glycerol
/\ C) Fatty acid D) Bilirubin
22. v) Lipoprotein I ipasc is activated by
/ J\)ApoJ\-I B)Ap60-10 0
iQ C,Apo E Dppo C- II
/
- 1024 z,

'\v
1/

Kana chur Instit ute of Medi cal Scien ces, Natek al

Depa rtmen t of Bioch emist ry
I MBB S (2023 -2024 )- Ill Intern al Asses smen t Exam
Date: 21.06 .2024
inatio n - Paper 2 \
Time : 9 AM- 12 PM
Max Mark s: 100
Durat ion: 3 hours
I. '\'rite your Name and Regis ter numb er on the answ
er bookl et.
2. Write neatly and legibl y.
3. Draw neat diagr ams wher ever neces sary.
4. Any malpr actice will be sever ely dealt with.
I. LON G ESSA YS:
2xl0= 20 mark s
1. A 5 year old child was broug ht lo the hospit al
by the paren ts who comp lained of
delaye d milest ones in the child. Inves tigatio ns reveal
ed high pheny lalani ne conte nt in
the blood .
a) Sugge st the proba ble diagn osis and name the
enzym e defec t in the above
disord er. (2)
l , b) Expla in the pathw ay for catab olism of pheny lalani
ne. (4)
"f-- c) Name the comp ounds respo nsible for the mous y
odour in the above condi tion. (1)
d) List the bioch emica l invest igatio ns in this disord
er. (2)
e) Name two comp ounds derive d from tyrosi ne. (1.)
S 1 2. What are
molec ular scisso rs? Give two examp les. Enum erate
the steps in recom binan t
DNA techn ology . Write two medic al applic ations
of recom binan t DNA techn ology .
(1+2+ 5+2)

II. SHOR T ESSA YS:

8XS= 40 mark s
3. A 45 year old man prese nted with severe back pain
and weakn ess. He had lost 7 kgs ,.r
in last three month s. X-ray show ed punch ed out lesion
s. Urine is positi ve for Benci ~ ~f>
Jones protei n.
'-"c a) Name the disord er
r, \ '\"'~
in the above case. (0.5)
/ b) Draw the electr ophor etic patter n that is obser ved
in this case. (1)
c) Name two plasm a protei ns and write their clinic al
signif icance . (2)
d) What are Bence -Jone s protei ns? How is it tested ?
(1 +0.5)
4. A 50 year old femal e prese nted with cold intole rance,
enlarg ed thyroi d, hoars eness of
voice, dryne ss of skin and exces s weigh t gain.
'}\ < (a) What is the proba ble diagnosis'? (I)
(b) Write labora tory findin gs in the above case. (2)
(c) List the tests for assess ment of' thyroi d functi ons.
(2)
'"'i ,, 5. Descr ibe DNA repair m~ch anism
wit.h suitab le examp les. Name one genet ic disord er
/ r cause d due to the defec t m DNA repair . (4+1)
,6. Class ify jaund ice. Write the serum and urinar
y findin gs in each type of jaund ice.
\/--- (3+2)
- '-"- '?. Expla in the secon dary struct ure of protei ns .
.,,,, ~ '8. Expla in immu nolog ical basis of vaccin e devel
opme nt. -?-~'r-
. J.. "9. Name and draw the struct ure of RNA, which
bring s amino acid to the site of protei n -\,
,,, synthe sis. List any two post transl ationa l modif icatio
ns. (3+2)
>., l 0. Enum erate the reacti ons of purine salvag
e pathw ay. Name the disord er that 1s
✓v assoc iated with this pathw ay and write it's clinic al featur
es. (3+1+ 1)

L
 III. SHORT ANSWERS: 10X3=30 marks
f'
11. Mention the c1dvantages of automation in clinical biochemistry laboratory.-
@vhat is homocystintiria? Name the enzyme defect in homocystinuria? What are its
t characteristic features. (1 +I+ I)
i 13. Explain the steps in polymerase chain reaction.
l 14. Ddine oncogene an? proto-oncog~ne? !-low i_s proto-oncogene activated? (1 +2)
.·,' • 15. Write the process of phase I det.ox1ficat1011 with one example.-
, 16. Draw ,111d label th~ cell cycle.
\ • 17. Name and explain any three barriers in doctor-patient communication. ,
~ 18. Expl~in the tests done to assess the renal tubular fonctions. ~
19. \Vhat nrc essentinl amino acids? Name them. (1+2)
20. What is lipid pc:-oxidation? Explain the role of antioxidants in scavenging reactive
\ oxygen species. ,(1 +2)

IV.MULTIPLE CHOICE QUESTIONS : l0xl=t0 marks

21. i) Tumor marker elevated in liver cancer is ----------------
\ , A) Prostate specific antigen -B') Alpha-fetoprotein
C) CA- l 25 D) B- HCG
21., ii) The closeness of the results of a quality control to its true value is termed as
~ ;r A) Sensitivity B) Speci~~ity
(J; Accuracy .0'Y Prec1s10n
21. iii) Southern blotting techniques is used for detection of-----------------
\ r J(JDNA B) RNA
C) Protein D) Lipoprotein
~- iv) The DNA fingerprinting is based on
A) Microsatcllite repeats B) LINES
C) SINES D) None of the above
21. v) Enzymes defect in acute intcm1ittent porphyria is
\ ~A) ALA synthase B) ALA dehydratase
___c;rtJroporphyrinogen I synthase D) Uroporphyrinogen decarboxylase
!]) i) Which of the following is an example for acute phase protein?
\ r A) Prothrombin 8) C- reactive protein
C) Lipoprotein D) Thyroxine binding globulin (TBG)
22. ii) Hypoalbumincmia is NOT SEEN in
, , 'A)_J;hronic liver disease v B) Nephrotic syndrome
; ef Hemoconcentration D) Protein energy malnutrition -
22. iii) l!Jlmunoglobulin which crosses placenta is
\. / --A) IgG B) IgA - tv-.'J\.ll/
, C) fgM - F'°'' D) IgD - 11
22. iv) W)rlch of the following antioxidant enzyme is selenium .dependent?
, A') Glut::nhione peroxidnse B) Glutathione reductase
/ C) NAD!'l f oxidasc D) Catalase
2-'J.. v) ·which of the following enzymes is respon~ible for immortality of cancer cells?
/A) Tclomcr;.isc B) RNA polymerase I
./ \· C) DNA polymerase D) Helicase
r
. . . . QP CODE: 1024 ~

Raj iv Gandh i Un1v.ers1ty of Health Scienc es, Kamat,1ka

MBBS Phase - I (CBME) Degree Examinat ion - 19-Aug-2 024

/ Time: Three Hours Max. Mark!s: 100

BIOCHEM ISTRY_ PAPER I (RS-4)

QP Code: 1024
(QP contains three pages)
Your answers should be specific to the questions asked
Draw neat labeled diagrams wherever necessary

.
LONG ESSAYS 2 x 10 = 20 Mark·s
1. A 24-year-old patient was brought to emergency departmen t in unconsciou s state. Past
history revealed that he was a known case of type 1 diabetes mellitus. His plasma glucost;~
on arrival was 580. Rothera's test with his urine was found to be positive. Provisional
diagnosis of diabetic ketoacidos is was made. Physician from emergency departmen t
suggested starting him on an insulin drip and administer ing one amp of bicarbonat e.
Monitoring of plasma glucose every hour was advised.
a) What is the acid-base disturbanc e of the above patient?
b) Mention the norm a I pH of blood.
c) Explain renal mechanism in maintenance of acid-base balance.
d) List two other clinical conditions causing above acid-base disturbance .

2. Mention the salient features of active site of an enzyme. Exp~in four factors affecting
enzyme activity.
,fir
SHORT ESSAYS 8 x 5 = 40 Marks
3. A 45-year-old HR manager complained of fatigue and tiredness. H~ also complained of
vague aches over his body and pains in the limbs. History revealed that he worked every
day in his air-conditio ned office for more than 10 hours and had limited exposure to
sunlight. After thorough clinical examinatio n, his Physician advised to check for Vitamin D3
levels. Report revealed to be Sng/mL (Reference range: 20 to 40 ng/mL).
a) Name the disorder caused by Vitamin D deficiency in adults.
b) Explain the biochemica l functions of Vitamin D.
c) What is Vitamfn D resistant rickets?
4. A 10-year-old girl presented with excessive tiredness, poor appetite, inability to
concentrate , difficulty in breathing on exertion and easy fatigability. On examinatio n,
there was pallor present. Laboratory tests revealed decrease in hemoglobin , ferritin and
MCV. Total Iron Binding Capacity (TIBC) and transferrin levels were elevated. Based on
above history and laboratory findings, iron deficiency anemia was made.
a) Mention two iron rich dietary sources.
b) Name two substances which decrease absorption of iron.
c) Explain mucosal block theory.

5. Define gluconeogenesis. Explain the pathway for formation of glucose from glycerol as
substrate.
6: Explain the metabolism of HDL. Substantia te why HDL is called as 'good cholesterol '.
7. Define dietary fibers. Give four examples of dietary fibers. Mention the beneficial effects of
dietary fibers.
8. Explain the metabolic adoptions during well-fed state.
9. Mention the coenzyme forms of riboflavin. list four biochemica l reactions in which
coenzyme forms of riboflavin participates .
10. Mention the significance of pentose phosphate Pathway and uronic acid pathway.

Page 1 of3
I 1025 S
QPCODl
.. dh'1 University of I-lealth Sciences, Kamatah
RaJ1v Gan
SHORT ANSWERS ·th one example to each 10 X 3 = 3;0 Ma\
dfport
1 wI •
11. Define syrnport an an ·t· n of collagen N
12. Mention the amino ac1·d composI 10 • ame two d.
isorders associated wit\
defective collagen. · n the clinical ·
How are sugar alcohols formed? Ment,o . . importance of sorbitol and mannvtol.
13. r osomes formed? Mention the clinical applications of liposomes
14. How are 1p mpl •
15. What are lipotrophic factors? Give four exa es of lipotrophic factors.
l6. What are high energy compounds? Give two examples and mention their significance.
17. Mention three biochemical functions of zinc.
18. Define obesity. Mention four health risks associated with obesity.
19. Define hyperkalemia. Mention two clinical conditions associated with hyperkalemia.
20. Name the bile salts and mention their functions.

Multiple Choice Question s 10 x 1 = 10 Marks

21 i) Km value of an enzyme is
A. Time required for completion of an enzyme catalysed reaction
B. Substrate concentration at half the maximum velocity
C. Half the substrate concentration at maximum velocity
D. Dissociation constant of enzyme-substrate complex
21 ii) Deficiency of which of the following vitamins results in excretion of xanthuren ic acid in
urine?
A. Pyridoxal phosphate A) P~f1D~t)Cine
B. Vitamin B12
C. Folic acid
D. Niacin
21 iii) Secretion of antidiuretic hormone (ADH) is stimulated by all the following EXCEPT
A. Increased sodium in serum
B. High osmolality of plasma
C. Inability to retain sodium
D. ECF volume contraction
E.
21 iv) Which of the following is a heteropoly haride?
A. Glycogen sacc
B. Heparin
C. Chitin
D. Inulin
E.
21 v)
Which of the following ,,
A. Albumin represents 'alkali reserve •
B. Phosphate
C. Hemoglobin
D. Bicarbonate
E.
22 i)
Marker enzyme for organelle I
A. Lactate dehydrogenas/sosorne iS
B. Ga_lactosyl transferase
C. Acid phosphatase
D. ATP synthase

Page 2 of 3

L
 QP coo E : 102~

sciences, I{arnataka
R ii) Maj : f~~_in adipuos!:~
• ~uersity
,ajiv Gandhi
of J:-Jealth
B. S iacylg lycer ol e is
c· phmgolipids
. Phospholipid
D. Cholesterol

22 iii) All of
B. the follow
Parat in argin
•
A. Lysin eh an~ ine
g increa seam·
the absorption of calcium from intes tine EXCE PT
C. Calcito~~~1d horm one mo acids
D. Calcitriol

22 iv) WhicB.h of
Chro . yro1d
the foll • ismco d". sis associated with secondary hype rlipid emia ?
owing
• Hyperth . n 1t1on •
A
me pancreatit·
CD. Hepat·t·I IS C infect i IS
. Nephrotic synd ro~~

22 v) Death cyato. cey~nic

A. due • ieitin
mhib poisonin g is
• as a result of
c· nid
Cyan~de hemo gl;b~ tochr ome oxidase
8 · Cyanide block
ing complex formation
. Cyanide inhibiti oxygen transport in blood
0 ng complex I of electron trans port chain

******
 , QP CODE : tOlS
Raliv Gandhi University ofH!!~~a~ci
:J en
MBBS Phase - I (CBME) Degre ces, Kamataka s
e tio n - 21 -A ug -20 24
Time: Three Hours Ma x. Marks: 10 0 Marks
BIOCHEMISTRY - PAPER
II (R S- 4)
Q.P. CODE: 1025
. s two pages)
{QP cont aan . ue stio ns asked
Your answers should be_ spe
cific
Draw neat, labeled diagrams toh t~! v~ r ne ces sar y
w e
LONG ESSAYS 2 x 10 = 20
Marks
was found to have yellow
l. ~Jes~~~ti~~ r::: ;1: ~~ serum total bilirubin 5.5 mg
ish dis col ora tio n of sk~•~·
Biochemical
/di and ser um dir ec t bihrub
in "4as 0.2
mg/:;- write biological refe
rence interval for serum
tot al bili rub in and ser um
bilirubin. direct
b) Explain the formation and
fate of bilirubin. .
. c) What is the biochemical .
basis for physiological Jaundi
c;t) Write the basis of giving ce? . . .
phototherapy in a case of phy
s1olog1cal J.aund1ce.
2. Describe the recombinant DN
A technology and me ntio n
its ap plic atio ns in me dic ine
.
SH OR T ESSAYS
3. A 45 year old business exe
cutive aft er partying in the
8 x 5 = 40 Ma rks
. up in the morning wit h sev nig ht wit h alc oho l and red
ere pain in the rig ht toe. It me at go t
and his biochemical invest was sw olle n. He rushed to
igations were as follows: a ho spi tal
serum uric acid: 10m g/d l. Se rum cre ati nin e: 0.9 mg
/di an d
a) Int erp ret serum uric acid
level in this patient
b) Explain the steps in the
formation of uric acid
c) Explain role of allopurino
l in this condition
4. A 60 year old man_ wh
o ha_s a. history of diabetes
Ne~hrolog_y OPD wit~ s":'ellm mellitus and hyp ert en sio n,
g 1_n the lower limb. His ser pre sen ted to
A d1agnos1s of chrome kidney um cre atin ine val ue wa s 3.8
disease was made . mg /di .
.a) Write biological referen
ce interval for serum creatin
. b) Explain creatinine cleara ine .
nce test.
5. Exptain mechanism of
action of steroid hormones.
6. Classify plasma protein
s. Describe the functions of
albumin.
7. Explain DNA replication
in prokaryotes.
8. Explain urea cycle. Mention
its regulation.
9. • Describe post-transcrip
tional modifications of RNA.
10. Explain secondary stru
ctural organisation of protein
.
SH OR T AN SW ER S
!;· ~efine Transamination and me
ntion the importance of Transa
• r~te the characteristics of gen
13. Wnte the role of p53 pro
etic code.
ion.
=
minat" 10 x 3 30 Ma rks
tein in cancer.
14. How is aspirin detoxified
in the body?
15. Wri~e three examples of
transmethylation reactions
l6. Name three biologlcall
17. Enumerate three cl Im .
Y •
portant nucleotides and wri
18. Name the antloxld astes te the ir functions
of lmmunoglobulins and wri
19. Mention any thre an te the ir function •
enzymes and write the bioche
20. Write the reactlo e pref-an mical reaction f s.
alytical variables encountere
ns purine salvage pathway.
O d in the clin• o an yon e of th
1
ca1 laboratory. ern •
 QP COD E : 1025

Rajiv Gandhi University of Health Sciences,10Ka mataka

X 1 = 10 Marks
Multiple Choice Que stion s
21 i) The following _are carcinogens gcE PI
• A. Affat bxin
B. Gamma rays
C. Benzopyrene
D. Actin omyc in
m is
21 ii) The class of antibodY most abun dant in the seru
A. lgG
B. IgM
C. lgA
D. IgE
proteins?
21 iii) Whic h of the following amin o acids is nQt found in
A. Arginine •
a. Histidine
c. Methionine
o. Citrurllne
follow ing is a nucleotide?
21 iv) Which of the
A. Aden ylic acid
B. Aden ine
C. Adenosine
D. Agm antin e
with
21 v) Bilirubin is made wate r soluble by conju gatio n
A. Litho choli c acid
B. Cholic acid
C. -Gluc uron ic acid
-
0. Chenodeoxychollc acid

22 i) Serotonin is synthesized from

A. Tyro sine
B. Tryp toph an
C. •Phenylalanine
D. Proline
tion of
22 ii) Western blott ing technique is used for the detec
A. DNA
B. RNA.
C. Proteins
D. Lipoproteins
22 i\i) N1 of pyrim idine ring is contributed by
A. Gfuta mine .
B. Aspa rtic acid
c. Glycine
D. Argin ine
ce tcfreradicals?
22 iv) Which of the follow ing cells s;loes not prbdu
A. Erythrocytes
B. Neutrophils
C. Basophils
D. Macrophages

22 v) Test used to detec t urob ilinog en in urine is

A. Fouchet's test
B. Hay's test
C. Ehrlic h's test
D. Roth era's test
*****
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