VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2022]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 3 hours Maximum Marks :100
SECTION – I
Q1. Multiple Choice Questions :- (2x10=20)
1) The respiratory acidosis is caused in which of the following?
(a) Pneumonia
(b) Prolonged starvation
(c) Intestinal obstruction
(d) Pyloric stenosis
2) Which of the following regulates lipolysis in adipocytes?
(a) Hormone- sensitive lipase
(b) Phospholipase A2
(c) Co-lipase
(d) Lipoprotein lipase
3) In competitive inhibition, which of the following kinetic effect is true?
(a) Decreases both Km and Vmax
(b) Increases both Km and Vmax
(c) Decreases Km without affecting Vmax
(d) Increases Km without affecting Vmax
4) Cyanide is poisonous because of:
(a) Inhibition of TCA cycle
(b) Combination with RB cell membrane
(c) Inhibition of cytochrome oxidase
(d) Inhibition of myoglobin
5) Which is not a part of normal adult haemoglobin molecule?
(a) Vinyl group
(b) Ferric iron
(c) Propionyl group
(d) Pyrrole rings
6) Normal reference values of anion gap ranges from:
(a) 18-20 meq/L
(b) 8-12 meq/1
(c) 1-10 meq/L
(d) 22-26 meq/L
7) Which is FALSE for Carboxyhemoglobin?
(a) It is depicted as HbCO
(b) It is excess carbon dioxide attached to Hb
(c) It has 200 times more affinity than oxygen
(d) It is life threatening
[ 1 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
8) Refsum's disease results from a defect in
(a) Alpha-oxidation of fatty acids
(b) Beta-oxidation of fatty acids
(c) Omega-oxidation of fatty acids
(d) Gamma-oxidation of fatty acids
9) Glucose and galactose are epimers. They differ structurally in orientation
of H and OH on:
(a) Cl
(b) C3
(c) С4
(d) C2
10) Physiological Jaundice in new-born is due to which of the following?
(a) Increased RBC turnover
(b) Immature liver cells
(c) Transient deficit of UDP Glucuronyl Transferase
(d) All of the above
Q2. Long Answer type Question :- (15x1=15)
What is Gluconeogenesis? Enumerate various substrates for
gluconeogenesis. Describe the process of gluconeogenesis from lactate as
substrate along with is regulation.
Q3. Answer the following :- (5x5=25)
(a) What is alpha oxidation? Mention its clinical significance. (2+3=5)
(b) Compare and contrast oxidative phosphorylation and substrate level (5x1=5)
phosphorylation.
(c) Describe how monoclonal antibodies are produced? Enumerate their (2+3=5)
applications.
(d) Define Metabolic alkalosis. Enumerate its causes. What are the (1+2+2)
compensatory mechanisms associated with it?
(e) Compare Competitive and Non Competitive Enzyme Inhibition along (5x1=5)
with suitable examples.
SECTION – II
Q4. Long Answer Type Questions :- (15x1=15)
A 50year old obese woman, presents with the complaints of excessive thirst
and hunger with increased urination. On physical examination, she
appeared dehydrated with dryness of tongue, and had mild rise in blood
pressure. The lab investigations showed fasting blood glucose 200 mg/dL,
postprandial blood sugar 350mg/dL, benedict's test (urine)- orange
precipitate.
(a) What is the most likely clinical diagnosis? (2)
(b) What is the diagnostic criteria for your diagnosis? Enumerate the other
laboratory investigations that you will do in this case? (3+2)
(c) What is the biochemical basis of polyuria, polydipsia and polyphagia in
this case? (3)
(d) What are the possible complications if the disease is not controlled? (5)
[ 2 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
Q5. Answer the following :- (5x5=25)
(a) Draw and Explain the oxygen dissociation curve in terms of cooperative (2+3=5)
binding of oxygen.
(b) What is oxidative stress? Discuss the role of free radicals in causation (1+4=5)
of atherosclerosis.
(c) What is the role of Carnitine in fatty acid oxidation? Explain its clinical (2+3=5)
significance.
(d) Enumerate the diagnostic markers of myocardial infarction (MI). (2+3=5)
Explain with diagram the pattern of rise of these markers following an
acute MI.
(e) What are phospholipids? Enumerate their types with examples. (1+2+2)
Describe their role in respiratory distress syndrome.
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[ 3 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2021]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 3 hours Maximum Marks :100
SECTION – I
Q1. Multiple Choice Questions :- (2x10=20)
1) Which one of the following is true about Glucose?
(a) It is a C4 epimer of galactose
(b) It is ketone and usually exists as furanose ring in solution
(c) It is produced from dietary starch by the action of amylase
(d) It is utilised in biological systems only in the L isomeric form
2) Classical galactosemia is due to deficiency of
(a) Hexoaminidase
(b) Glucocerebrosidase
(c) Sphingomyelinase
(d) Galactose-1-phosphate Uridyltransferase
3) Gluconeogenesis mainly occurs in
(a) Liver
(b) Brain
(c) Muscle
(d) Heart
4) Which of the following statements is true about fetal haemoglobin?
(a) It has sigmoid O2 saturation curve
(b) Its concentration increases in thalassemia
(c) It binds to 2,3 BPG with higher affinity than HbA
(d) It consists of 2 and 2 polypeptide chains.
5) If 2,4 dinitrophenol is added to tightly coupled mitochondria that are
actively oxidising succinate, which of the following statement is true?
(a) Electron flow will continue but ATP will not be synthesized
(b) Electron flow will continue but ATP will be synthesized
(c) Electron flow will cease but AT will be synthesized
(d) Both electron flow & ATP synthesis will stop
6) In competitive inhibition, which of the following kinetic effect is true?
(a) Decreases both Km and Vmax
(b) Increases both Km and Vmax
(c) Decreases Km without affecting Vmax
(d) Increases Km without affecting Vmax
7) Cyanide is poisonous because of:
(a) Inhibition of TCA cycle
(b) Combination with Red Blood Cell membrane
(c) Inhibition of cytochrome oxidase
(d) Inhibition of myoglobin
[ 4 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
8) The main function of pentose phosphate pathway in humans is:
(a) ATP production
(b) NADH production
(c) Carbon dioxide production
(d) NADPH production
9) The most important physiological buffer in the blood is:
(a) Haemoglobin buffer system
(b) Bicarbonate buffer
(c) Phosphate buffer
(d) Acetate buffer
10) Antibody involved in type I hypersensitivity
(a) IgD
(b) IgE
(c) IgA
(d) IgG
Q2. Long Answer type Question :- (15x1=15)
Outline the pathway of heme synthesis with the help of a flowchart.
Explain briefly its regulation. Discuss Acute Intermittent Porphyria.
Discuss briefly the biochemical basis treatment of porphyrias.
Q3. Answer the following :- (5x5=25)
(a) Explain the role of 2, 3 Bisphosphoglycerate on oxygen binding
characteristics of haemoglobin with the help of a diagram.
(b) Compare and contrast oxidative phosphorylation and substrate level
phosphorylation with suitable examples.
(c) Define Metabolic alkalosis. Enumerate its causes. Describe the
compensatory mechanisms associated with it?
(d) Discuss Competitive inhibition of enzymes along with diagrams of
saturation curves and suitable examples.
(e) Explain briefly the importance of antigen presenting cells in immune
reactions
SECTION – II
Q4. Long Answer Type Questions :- (15x1=15)
A 45 year man felt sudden chest pain at 4 am in the morning. Initially
moderate in intensity, the pain gradually became increasingly severe, and
soon he felt severe constriction around the chest which caused difficulty
in breathing. The pain also radiated to the left arm. The patient was rushed
to the nearest nursing home. Chest X-ray and ECG were done along with
other blood investigations. ECG showed changes including ST elevation.
Answer the following questions with relevance to the above clinical case:
(a) What is the most probable diagnosis ? (2)
(b) What biochemical tests would be of relevance in this case to confirm (3)
the diagnosis?
(c) Discuss the significance of isoenzyme estimation in this case? (5)
(d) Discuss the life style modifications that may be useful for this patient. (5)
[ 5 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
Q5. Answer the following :- (5x5=25)
(a) Discuss briefly the free radical scavenging enzymes.
(b) Define phospholipids. Enumerate their different types and describe
briefly their significance.
(c) Discuss briefly the applications of monoclonal antibodies.
(d) Explain briefly how Kreb's cycle is amphibolic in nature.
(e) Explain the post translational modifications during collagen synthesis.
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[ 6 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2020]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 3 hours Maximum Marks :100
SECTION – I
Q1. Multiple Choice Questions :- (2x10=20)
1) Glucose and galactose are epimers. They differ structurally in orientation
of H and OH on:
a. C1
b. C3
c. C4
d. C2
2) Which of the following regulates lipolysis in adipocytes?
a. Hormone- sensitive lipase
b. Phospholipase A2
c. Co-lipase
d. Lipoprotein lipase
3) In competitive inhibition, which of the following kinetic effect is true?
a. Decreases both Km and Vmax
b. Increases both Km and Vmax
c. Decreases Km without affecting Vmax
d. Increases Km without affecting Vmax
4) Cyanide is poisonous because of:
a. Inhibition of TCA cycle
b. Combination with RB cell membrane
c. Inhibition of cytochrome oxidase
d. Inhibition of myoglobin
5) Which is not a part of normal adult haemoglobin molecule?
a. Vinyl group
b. Ferric iron
c. Propionyl group
d. Pyrrole rings
6) Biotin is involved in which of the following types of reactions?
a. Carboxylation
b. Hydroxylation
c. Oxidation
d. Deamination
[ 7 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
7) The two nitrogen atoms in urea arise from:
a. Ammonia and glutamine
b. Glutamine and aspartic acid
c. Glutamine and glutamic acid
d. Ammonia and aspartic acid
8) The most important physiological buffer in the blood is:
a. Haemoglobin buffer system
b. Bicarbonate buffer
c. Phosphate buffer
d. Acetate buffer
9) Keshan’s disease is associated with the deficiency of:
a. Molybdenum
b. Manganese
c. Selenium
d. Sulfur
10) Which vitamin is responsible for the transfer of one carbon moieties from
one molecule to another?
a. Biotin
b. Vitamin C
c. Riboflavin
d. Folic acid
Q2. Long Answer type Question :- (15x1=15)
Define Ketone bodies. Explain their synthesis and utilization in the body.
Discuss the conditions in which ketoacidosis occurs along with their
biochemical basis.
Q3. Answer the following (Any FIVE) :- (5x5=25)
(a) Explain why Glutathione is important for RBC membrane integrity.
(b) Describe the sources, biochemical functions and deficiency
manifestations of Vitamin D.
(c) Compare and contrast oxidative phosphorylation and substrate level
phosphorylation.
(d) Describe how monoclonal antibodies are produced? Enumerate their
applications.
(e) Define Metabolic acidosis. Enumerate its causes. What are the
compensatory mechanisms associated with it?
(f) Discuss Non-competitive inhibition of enzymes along with diagrams of
saturation curves. Explain with suitable examples.
[ 8 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
SECTION – II
Q4. Long Answer Type Questions :- (15x1=15)
A 40year old woman, weighing about 80 kg, came to the OPD, complaining
of weakness, lethargy and increased hunger for the last 2-3 months. She
also noticed that she felt very thirsty. In the night, she gets up 4-5 times
for urination. On physical examination, she appeared dehydrated with
dryness of tongue, and had mild rise in blood pressure. The lab
investigations showed fasting blood glucose – 200 mg/dL, postprandial
blood sugar- 350mg/dL, benedict’s test (urine)- orange precipitate.
(a) What is the most likely clinical diagnosis?
(b) What is the diagnostic criteria for your diagnosis? Enumerate the other
laboratory investigations that you will do in this case?
(c) What is the biochemical basis of lethargy, polyuria and polydipsia in
this case?
(d) What are the acute and chronic complications associated with this
condition?
Q5. Answer the following :- (5x5=25)
(a) Describe sickle cell anaemia. Explain its biochemical basis.
(b) Enumerate the properties of cytochrome P450. Discuss its importance
in drug interactions with suitable examples.
(c) Describe the clinical features of alkaptonuria and enumerate the
investigations done its diagnosis. Justify the excretion of black colored
urine is excreted in alkaptonuria.?
(d) Explain briefly how Kreb’s cycle is amphibolic in nature.
(e) Enumerate the differences between Proteoglycans and Glycoproteins
with suitable examples.
************
[ 9 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2019]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 2 hours Maximum Marks :40
SECTION – I
Q1. Write briefly on :-
(a) Cardiac Isoenzymes with their clinical significance. (5)
(b) Type I Hypersensitivity (5)
Q2. Write short notes on :-
(a) Lysosomal markers (2.5)
(b) Enumerate the disorders associated with defective extracellular matrix. (2.5)
Q3. How are monoclonal antibodies produced? Write their applications. (5)
SECTION – II
Q4. Explain :-
(a) Acute Phase Reactants (5)
(b) ELISA (5)
Q5. How is heme synthesised in the body? Explain the regulation of this pathway. (10)
OR
Explain the various types of enzyme inhibition with examples. (10)
************
[ 10 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2018]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 2 hours Maximum Marks :40
SECTION – I
Q1. Explain briefly :- 2.5x4=10
(a) Gluconeogenesis is not a simple reversal of glycolysis.
(b) There is hemolysis in glucose-6-phosphate dehydrogenase deficiency.
(c) Role of essential fatty acids
(d) Isomerism in Carbohydrates.
Q2. Write briefly on :- 2.5x2=05
(a) Cardiac Isoenzymes with their clinical importance.
(b) Von-Gierke’s Disease
SECTION – II
Q3. Write short notes on the following :- 2.5x2=05
(a) Secondary structure of proteins with examples
(b) Various functions of mucopolysaccharides in body
Q4. Draw a well-labelled diagram of fluid mosaic model of plasma membrane. 2+3=05
Discuss the various membrane transport mechanisms briefly.
Q5. How is glycogen catabolised in the body? Explain the regulation of this 10x1=10
pathway.
OR
Discuss the various types of enzyme inhibition with examples. 10x1=10
************
[ 11 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2017]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 2 hours Maximum Marks :40
SECTION – I
Q1. What is sickle cell anaemia? Explain the biochemical basis of sickle cell 5x1=05
crisis.
Q2. (a) What is respiratory distress syndrome? What is its biochemical basis ? 5x1=05
(b) What is the difference between coagulation and denaturation?
Q3. What are isoenzymes? Give examples. Write briefly on isoenzymes used to 5x1=05
diagnose Myocardial Infarction.
Q4. Draw a labelled diagram of fluid mosaic model of membrane. 5x1=05
SECTION – II
Q5. Write briefly :- 5x1=05
(a) Bohr Effect
(b) Acute Phase Reactants
Q6. Write briefly :- 5x1=05
(a) Galactosemia
(b) Von-Girke’s Disease
Q7. What are glycosaminoglycans? Give examples and mention their functions 10x1=10
in human body.
OR
Explain how Kreb’s cycle is amphibolic in nature. 10x1=10
************
[ 12 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
VARDHMAN MAHAVIR MEDICAL COLLEGE AND SAFDARJUNG HOSPITAL
M.B.B.S. Batch [2016]
SUMMATIVE ASSESSMENT I
BIOCHEMISTRY
Duration : 2 hours Maximum Marks :40
SECTION – I
Q1. Write a short note on factors affecting enzyme activity. (5)
Q2. Describe the secondary structure of proteins. (5)
Q3. What are phospholipids? Give examples. Enumerate their functions. (5)
Q4. Discuss briefly on osmosis, diffusion and surface tension in relation to human (5)
body.
SECTION – II
Q5. What are isoenzymes? Write briefly on isoenzymes of LDH. (5)
Q6. Write briefly on :- (5)
(a) Antioxidants
(b) What is pH? Enumerate the buffers used in regulation of blood pH.
Q7. Draw a labelled diagram of fluid mosaic model of plasma membrane. Describe (10)
briefly the various transport mechanisms.
OR
Mention various polysaccharides and their significance in human body. (10)
************
[ 13 ]
COMPILED BY AARYAN TYAGI (2019 MBBS BATCH, VMMC AND SAFDARJUNG HOSPITAL)
