DEAD-box 헬리코아제 20 , 보석 관련 단백질 3 (GEMIN3)로도 알려진 ATP 의존성 RNA 헬리코아제 DDX20은 인간 에서 DDX20 유전자에 의해 암호화 된 효소 다. [5] [6]
함수 보존 모티브인 Asp-Glu-Ala-Asp(DEAD)가 특징인 DEAD 박스 단백질은 Putative RNA 나선형 이다. 그것들은 변환 개시, 핵 및 미토콘드리아 스플라이싱, 리보솜 및 스플라이소솜 조립과 같은 RNA 2차 구조의 변경을 포함하는 많은 세포 과정에 관여한다. 이들의 분포 패턴으로 볼 때, 이 가족의 일부 구성원은 발생 , 정자생식 , 세포 성장 및 분열에 관여하는 것으로 여겨진다. 이 유전자는 ATPase 활성이 있고 운동신경 (SMN) 복합체 생존 의 구성요소인 DEAD 박스 단백질을 인코딩한다.[6] SMN은 척추 근위축성 유전자 산물로, RNP에서 SMN 복합체의 기능에 촉매 역할을 할 수 있다.[6]
생물학적 시사점 이전의 연구에서는 DDX20이 간세포암 에서 종양 억제제 역할을 하고 유방암 에서 종양 촉진제 역할을 할 수 있다는 것이 밝혀졌다. DDX20 결핍은 마이크로RNA의 NF-κB 억제 작용을 손상시켜 NF-κB 활동을 강화하며, 마이크로RNA 기계 구성 요소의 조절 오류도 다양한 인체 질환의 병원생식에 관여할 수 있음을 시사한다.[7] 간종양 억제기 역할을 하는 miRNA-140과 같이 DDX20의 결핍으로 miRNA-140 기능이 손상되면 miRNA의 이러한 후속 기능 손상은 간상화생식으로 이어질 수 있다. 마찬가지로 DDX20은 NF-118B 경로를 통해 전립선암 (PCA)의 진행을 촉진할 수 있다.[8] [9] 임상 기반 연구에서 양성 DP103/NF-170B 피드백 루프는 침습성 유방암에서 구성성 NF-170B 활성화를 촉진하고 이 경로의 활성화는 암 진행 및 항암화학요법 내성 획득과 관련이 있다는 것이 관찰되었다. 그것은 DP103이 유방암 치료의 치료 목표로서 잠재력을 갖도록 만든다.[10]
상호작용 DDX20은 다음과 상호 작용하는 것으로 나타났다.
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PDB 갤러리
2oxc : 인간 DEAD-box RNA 헬리코아제 DDX20, ADP 복합체 DEAD 도메인