ACOMPI

LATI
ONOFBCH201&BCH203PASTQUESTI
ONS

BCH201(
2010/
2011)
PART1
1a. Der
ivet
heHENDERSONHASSELBALCHequat
ion

1b. Whati
sthepHofasol
uti
onf
orwhosehy
drogeni
nconcent
rat
ioni 2×10–4mol
s3. /l
?

2a. Whati
sthepHofasol
uti
onwhosehy
doxi
dei
onconcent
rat
ioni 0×10–4mol
s4. /l
?

2b. Gi
vet
woexampl
eseachofDi
prot
icAci
dsandMonopr
oti
caci
ds

PART2
1. Li
standex
plai
nwi
thr
elev
antequat
ions,
dif
fer
entr
eact
ionsofmonosacchar
ides

2. Descr
ibet
hev
ari
ouscl
assesofcar
bohy
drat
es.

PART3
1. Wr
it
econci
sel
yoncel
lor
ganel
l
eswi
thr
espectt
othei
rst
ruct
uresandf
unct
ions

2a. Dr
awst
ruct
uresoft
hef
oll
owi
ng:

-
Sphi
ngomy
eli
n

-
Phosphat
idy
let
hanol
ami
ne

-Phosphat
idy
lser
ine

-
Tri
stear
in

-
Chol
est
erol

2b. St
ate5f
unct
ionsofl
i
pidsi
nmammal
s

PART4
1a. Dr
awt
hest
ruct
ureoft
hispent
a-pept
ide:
.E-
A-G-
L-E

1b. Wr
it
eont
hev
ari
ousst
ruct
ural
lev
elsofpr
otei
ns.

2a. Enumer
atet
hev
ari
ousf
act
orsaf
fect
ingenzy
meact
ion.

2b. Tost udythedependenceoftherateofanenzyme-catalysedreacti

ononthesubstr
ate
concentrati
on,
aconstantamountofenzy meisaddedt oaser i
esofreacti
onmixtur
es
containi
ngdiff
erentconcent
rat
ionsofsubstr
ate.Considersuchanexper i
mentinwhicht
he
i
nit
ial r
atesint
het abl
ebelowwer eobt
ainedattheindi
cat edconcentr
ati
on.
[
S](
mol
/l
). Vel
(mol
/mi
n)

0×10–1
2. 60

0×10–2
2. 60

0×10–3
2. 60

0×10–4
2. 48

5×10–4
1. 30

3×10–5
1. 15

a. Ony
ouranswerbookl
et,
sket
cht
hegr
aphofVel
.agai
nst[
S] concent
rat
ion

b. Fr
om t
hegr
aphdet
ermi
net
heKm andt
heVmaxf
ort
hisr
eact
ion?

c. Whyi
sVconst
antabov
esubst
rat
econcent
rat
i 0×10–3M?
onsof2.

d. Whati
stheconcent
rat
ionoff
reeenzy 0×10–2M subst
meat2. rat
econcent
rat
ion?

PART5
1a. Wr
it
eshor
tnot
eont
hef
ourl
evel
sofst
ruct
urei
npr
otei
ns

1b. Gi
veanexampl
eandt
hest
ruct
ureoft
hef
oll
owi
ng:

-Aminoaci
dsthatabsorbsat280nm
-Aminoaci
dswithanimi nogroup
-Aminoaci
dswithhydrogenbondi ngcapaci
ty
-Aminoaci
dswithtwochi r
alcarbon.
2a. Howar
eenzymesdif
ferentf
rom mostcat aly
stsusedinsy
nthet
icchemi
str
y?

2b. Highl
i
ghtthemeri
tsanddemer
it
sofenzy
menomencl
atur
ecr
eat
edbyt
heI
nter
nat
ional
Commi ssi
ononenzymes.

2c. Anenzy mewithKm of2. 4×10–4wasassay edatt hefoll

owingsubstr
ate
2 –5 –4 –3
concentr
ati
ons:(
a)2×10M ( b)6.3×10 M ( c)10 M ( d)2×10 M and0.05M.Thevel
oci
ty
–1 -1
observedat0.
05M was128nmol es×l
it
re ×mi n .Cal culat
etheini
ti
alvel
oci
ti
esatt
heother
substr
ateconcentr
ati
ons.

PART6
1a.Assesst
heaccept
edmodel
ofanamedmembr
ane.

2.Wr
it
eshor
tnot
eonanyf
ouroft
hef
oll
owi
ng:

-Bi
ogenesis
-Dol
ichol
-Cl
athri
ne
-Symport.
 BCH201(3uni
ts)
(
2016/
2017)HARMATTANSEMESTER
1a. Discussthefactor
sthati
nfl
uencethefunct
ionoft
heact
ivesi
teofanenzy
meandenumer
ate
thecharact
erist
icsoft
hesit
e ( 10mrks)

1b. TheATPi
stheener
gyexchangecur
rency
,di
scuss. (
5mr
ks)

1c. Descr
ibet
heuni
quer
oleoftheenzy
mecommissi
onnumbercompar
edwi
tht
hei
mpor
tanceof
ot
herenzy
menomenclat
ureusi
nganexampl
e. (10mrks)

2a. DefinepHinquantit
ativ
eterms (1mr k)
2b. Explainwhynotall
liqui
dshaveapHv alue. ( 2mrks)
2c. Statefiv
eappli
cati
onsofpHi nev
erydayl i
fe. (5mrks)
–4
2d. Calculat
ethepHoft hefoll
owi
ng(i
)5×10 M HCl (
i)7×10–5NaOH(
i i
ii
)0.
04M HCl
(iv
)3M KOH.
(6mr ks)
2e. Useequat i
ononlytoexpresst
hedissociati
onconstantofaweakaci
d. (1mrk)

2f
.(i
)whati sthepHofali
tresoluti
onwhichwaspr
eparedbydissol
ving0.
02molesofacet
icaci
d
(HOAc;pKa=4.8)i
nwat er
, (
ii
)if0.
008mol
esofconcent
ratedsodi
um hy
droxi
dewaslater
addedforthi
ssol
uti
on,cal
culatethenewpH. (5mrks)

3a. Li
stt
hecl
assesofl
i
pidsandgi
veex
ampl
esoft
hei
rappl
i
cat
ions. (
3mr
ks)

3b. Howdochai
nlengt
handdegr
eeofsat
urat
ionaf
fectphy
sical
proper
ti
esofl
i
pids?
..
..
(5mr
ks)

3c. Drawthestr
ucturesofthef
oll
owi
ng:(i)
1-st
earoy
l,
2-l
inoleoy l
,3-
palmi
toy
lgly
cer
ol(
ii
)
9,
12,
15
Phosphat
idy
lseri
ne(ii
i
)Phosphat
idy
lseri
ne(iv
)18:3(
∆ ),
(v)chol
est
erol
.

3d. Br
ief
lyexpl
ainwhyt
ransunsat
urat
edf
att
yaci
dsshoul
dbemi
nimi
zedi
nourdi
et.(
5mr
ks)

4a. Br
ief
lyout
li
nehowacel
lcanbesepar
atedt
othei
rdi
ff
erentcomponent
s. (
15mr
ks)

4b. Givet
helistoft
hedi
ff
erentcl
asshi
ghener
gycompoundandexampl
esofspeci
fi
ccompound.
(10mrks)

5a. All
carbohydr
atesar
ecomposedofmonomer scal
l
edmonosacchar
ides,highl
i
ghtthestr
uct
ure
ofthefol
lowi
ngmonosacchar
ides:
glucose,
galact
ose,
fruct
ose,
andribose. (10mrks)

5b. Wri
tebr
iefl
yonthetwomajortypesofst
oragepol
ysacchar
ides,
not
ingt
hei
rdi
ff
erences,
and
si
mil
ari
ti
esint
erm ofst
ruct
ures. ( 15mr ks)

6a. Descr
ibet
hest
ruct
ural
classi
fi
cat
ionofAmi
noaci
ds,
wit
htwoexampl
eseach. (
12mr
ks)

6b. Drawthest
ructur
esofthef
oll
owi
ngAmi
noaci
ds:
val
i
ne,
tyr
osi
ne,
cyst
eine,
lysi
neand
aspar
tat
e. ( 8mrks)

6c. Namef
ivenon-
prot
einAmi
noaci
dsandt
hei
rfunct
ions. (
5mr
ks)

7. Wr
it
eshor
tnot
eonanyf
ouroft
hef
oll
owi
ng:

a.Fluidmosai
cmodel
b.Dichol
c.Receptormediat
edendocytosi
s
d.Sy mport
e.Na+- -
K+ATPase. (
6mr kseach)
8. Doadiscour seonaspeci
fi
cwaterorfatsolubl
evit
aminwitht
hefoll
owinggui
des
(a)atl
eastfoursour
ces(b)f
ourfuncti
ons(c)str
uctur
e(d)def
ici
ency. (6mrkseach)
 BCH203(
2UNI
TS)
2013/
2014HARMATTANSEMESTER
SECTI
ONB
I
nst
ruct
ions(
answert
hreequest
ionsonl
y)

1a. Wit
htheai
dofadi
agr
am,
showt
hef
ormat
ionofhy
drogenbondsbet
weent
hebasepai
rsi
n
DNA.

1b. Bri
efl
y,hi
ghl
i
ghtt
hecont
ri
but
ionoft
hef
oll
owi
ngsci
ent
ist
stot
hedev
elopmentofmol
ecul
ar
genet
ics

i
.Erwi nChargaff
i
i.Rosali
ndFr anklinandMaur i
ceWilkins
i
ii
.JamesWat sonandFr ancisCrick
i
v.Al f
redD.Her seyandMar thaChase
v.Oswal dT.Av ery,Coli
nMacLeodandMacl ynMcCar
ty
1c. Inacer
tainDNAchemi calanalysi
s,thepercentagecomposi
ti
onofG i
sol
atedwasest
imat
ed
as70%,ifthetotal
py ri
mi dineswere2100, calcul
ate

i
.Percentagecompositi
onofA, TandC
i
i
.thetotalpuri
nebases
i
i
i.thetot
al numberofA,G,TandC
i
v.Thelengthofthi
sDNAmol ecul
e
v
.Thenumberofhel icaltur
nsi nthi
sDNAmolecul
e
v
i.thenumberofbasepai rs(bp)inthi
sDNAmolecul
e.

2a. Di
scussthest
epwisest
ageint
heDNArepl
i
cat
ion
2b. Hi
ghl
ightt
hedi
ffer
encesbet
weent
heDNAandRNA

3. Di
scusst
hev
ari
oust
ypesofMut
ati
on,
andhi
ghl
i
ghtt
hesi
gni
fi
canceofMut
ati
on.

4a. Intowhichtwogroupscantheni
tr
ogen-contai
ningbasesthatf
orm DNAandRNAbe
classi
fi
ed?.Whatisthecr
it
eri
onusedinthatcl
assif
icat
ion?

4b. Bacter
iaareprokar
yoti
ccel
ls,i
.e,
theydonothaveamembr ane-
del
imi
tednucl
eus,
Eukar
yot
es
havecell
swithadelimi
tednucl
eus.Whereintheset
ypesofcell
scanDNAbefound?

5a. Whatist
henumeri
crel
ati
onbet
weenpy
rimi
dineandpur
inebasesi
ntheDNAmol
ecul
e?I
sthat
r
elat
ionval
i
dinRNAmolecul
e?

5b. Whi
cht
ypeofchemi
cal
bondmai
ntai
nst
hepai
ri
ngofeachchai
nint
heDNAmol
ecul
e?
 BCH203(
2UNI
TS)
2017/
2018HARMATTANSEMESTER.
I
nst
ruct
ions:AnswerOnl
yFourQuest
ions

1a. Out
li
net
hedi
ff
erencesbet
weenDNAandRNA. (
5mr
ks)

1b. DNAi
sthepol
ymerofdeoxy
ribonucl
eot
ides,
discuss. (
10mr
ks)

1c. Expl
aint
het
hreemaj
orf
ormsofDNA. (
10mr
ks)

2a. Whatar
enucl
eot
ides?St
atet
hedi
ff
erencesbet
weennucl
eosi
desandnucl
eot
ides. (
8mr
ks)

2b. Nameanddr awt

hestruct
uresoft
hef
ourmaj
ordeoxy
ribonucl
eot
ides5'
-
monophosphat
esand
r
ibonucl
eosi
de5'
-monophosphat
es. (17mrks)

3. Dist
inguishamongt
hesedif
ferentsequenci
ngtechni
ques,
basi
c,advancedandnext
generati
oninter
msofexample,ease,costofoper
ati
onandeff
ici
ency. (25mrks)

4a. Highl
i
ghtt
heconsequencesofthesyst
em ofconj
ugateddoubl
ebondsoft
hehet
erocy
cli
c
r
ingsofni
tr
ogenbasesonnucleicaci
ds. ( 10mrks)

4b. Dr
awt
hest
ruct
uresoft
hef
oll
owi
ng:

i
.sy
nGuanosi
ne

i
i
.ant
iUr
idi
ne

i
i
i.Deoxy
adenosi
ne5'
-
monophosphat
e

i
v.N2-
Met
hyl
guanosi
ne

v
.5-
Met
hyl
cyt
idi
ne. (
3mr
kseach)

5a. Wr
it
eont
heel
ongat
ionst
ageofpol
ypept
idesy
nthesi
s. (
8mr
ks)

5b. Whati
sthesi
gni
fi
canceofShi
ne-
Dal
ganosequenceatt
he5'
-
endofpr
okar
yot
icmRNAs.
(
5mrks)

5c. Gi
ve,i
ntabul
atedfor
m,t
hreei
nhi
bit
orsofpr
okar
yot
icpol
ypept
idesy
nthesi
sandt
hepr
ocesses
af
fect
ed. (12mr
ks)
 BCH201(
3UNI
TS)
2017/
2018HARMATTANSEMESTER
I
nst
ruct
ions:AnswerAnyFourQuest
ions

1. Wr
it
eshor
tnot
esonanyf
ouroft
hef
oll
owi
ng:

a.Fl
uidmosai
cmodel
.(b)Dol
i
chol(
c)Receptormedi
atedendocy
tosi
s(d)Na+-
--
-K+ATPase(
e)
Symport
. (6andonequar termarkseach)

2a. Wi
thexampl
es,
li
stt
hedi
ff
erentcl
assesofl
i
pidsandst
atet
hei
rimpor
tance.(
5mr
ks)

2b. Li
standex
plai
ntwof
act
orswhi
chaf
fectphy
sical
proper
ti
esofl
i
pids? (
5mr
ks)

2c. Drawthestructuresofthef
oll
owi
ng:(1-
stear
oyl,
2-l
inol
eoyl
,
3-pal
mitoyl
glycer
ol.(ii
)
Phosphati
dylseri
ne(ii
i
)Phosphat
idy
lseri
ne(i
v)18:3(∆9,
12,
15),(
v)Phosphati
dylseri
ne
ever
ythi
ng

2d. Br
ief
lyexpl
ainheal
thi
mpl
i
cat
ionoft
hel
ongi
ntakeoft
ransunsat
urat
edf
att
yaci
d. (
5mr
ks)

3a. Discussthei
mport
anceoft
heenzy
mecommissi
onnumbercompar
edwi
thot
herenzy
me
nomenclatur
eusi
nganexampl
e. (10mr
ks)

3b. Descri
bethef
actorsthatdefi
nedthef
unct
ioni
ngoft
heact
ivesi
tesofanenzy
meand
el
ucidat
ethecharact
erist
icsoft
hesit
e (15mr
ks)

4a

i
.Whi
choft
heAmi
noaci
dshav
eabasi
cchai
n?

i
i
.Whi
choft
heAmi
noaci
dspossessesachar
geof+1atpH7.
0?

i
i
i.Whi
choft
heAmi
noaci
dsi
sdesi
gnat
edbyt
hesy
mbol
K?

i
v.Whi
choft
heAmi
noaci
dsi
sint
hesamecl
assaspheny
lal
ani
ne?

v
.Gi
vet
henameoft
hef
ourAmi
noaci
ds

v
i.Namet
heot
herAmi
noaci
dsoft
hesamecl
assasZ

4b. Enumer
atef
iveoft
hef
unct
ionsofpr
otei
nsandgi
veappr
opr
iat
eexampl
es. (
15mr
ks)

5. Showt
hedi
ff
erencesbet
weent
hesecl
assesofsugar
s (
25mr
ks)

i
.Al
dot
ri
oseandKet
otr
iose

i
i
.Enant
iomer
sandEpi
mer
s

i
i
i.Homopol
ysacchar
idesandHet
eropol
ysacchar
ides

i
v.St
orageandst
ruct
ural
sugar
s

v
.Amy
loseandgl
ycogen.
Ol
uay
oFi
say
oFel
ici
ta
P.
K.A#Pr
imeRose wi
shesYOUt
hev
erybest
!!
!
#Toget
her
_We_
Can
#Rev
olut
ion