CM 103
—.
a Beochemtstry =
smaller melecule.+ energy »
ore prctefn —+ die ,
Ba ene cease
» pNAm = RN
gee mn) Reena ustphien)
ortega
Ssgntnece -
(Hranalation)
eeneeeess Y
cential degra
Cemctetn synthesis)
- « Carnlochyadortes:
Ly nydeoted weston 4 cosbon.
glucose. (wr Jalyeeraldehwe
iy %
(Simplest a cosrbohybrates)
Essentials of Atock Afochemist Ty.
chem. be urithin cell.
angans > Hesuec + cells Le metabolttee.=
: metabolfem = sienemteal
pours, uuithts cell « ge 20
wiochemtcal P. eam Others.
ae A TUES. (etabaltem) iGSanee Wee cae
peta cabled B30 459 prag
mm Rrabolfem, * 4 ms &S. @) @
Shey met
te eee Crretetny “) phone 9264331436.
amino aclds. Pack Lentnget-
4 disease = upregulation / downregutotion Of any pouecess a bodig.
we cOUs eae though Uhemftce uhemitcal mesenger,
“(retabelttey.
control pegina- = (genetic expuessfon]|, ure cariouy ba ise
ee gon
i re ee Anecogh gene - Parutrucn’s dét
hour info is casucfed.
Ss oncp expriesserthe Info that wet ane casurpiog
S0 ge
-ow
a a n— Or a= Ketone.
Bee oi dre ert
ne or
oH
BOF |
noasibee
190.
end | Enter mediate
of mm
has protetas.
4
| wrecepter.tian channels.
—cent
ter membrane = tepid layer
ue a A
7 eN'- ECE
womreon TE
u
w god poscticle -
r\c9}23.ee i ae
yeu Be c cammein fn cewsbo hyde. +han
Lee agelic SY: cenveuien leade to stability te
ogo due
= gee, aldehyde fs masked
ae at Chidden), 2, move sible.
(es attacks,
onset g
a
Bod - qucapyranese,
a Anemectic CAMDEN »
e ReduLcthg us nen reducing Sugan
pidelnyde + RN = amine - e
a \ j og
Rent RCo: HT -t- a
ee peaterpeeg le ee
OnWeR NEHER. ONR
exe phenyl Mydaene i :
ACOH step
Cog NH NH +R
: \
Ne RK
RCH =N—NH-Ph,
eta
oer ema 3
AHO. (same ‘rex?).
ABO ‘blood ps.CASE“T Discomeny of anti ~ Hw doug. (Pecotense wah
1S yr
Ame = 'S ye
ee pat Set
steve development’ cost, 'S—2 biticn F
Fguccess = y An
aes dint! Nate Ges
s Gometiny net en ;
x < oe Es)
& iden featven trial
problem. : ; oa
Cdrsease), ad ¢
D-identity the tanget : .
Creoles dasget e bey
\D. se cau) f c
ue meutghianal, ’
fPo-Ubtne Studeg (OUESide te cell)
then Study en, Smallced qreup
Fhe ED} menkey ete.
men. cUntcad Lest,
— BNA uu Ht
NE euarrtndd Fence poe .
Tyson (seedy). > by | nen-Ulustng |
HIV = RNA st
cement = wird vi Sly Retro
Fearn es uiyus.
1 RNA).
FoR HIVeattack &
Steps
@ Attachments ef utyus,
waith hest CEU urhich
conten Ust vieceptcs.
i& infect” > Co count less.
Co, cba (a ptetecr)
Craxget tox uulyud)@). neu wires BA Interac unith Aoct BWA.
Irtegraticn ;
Engyme= Potegase
|G) Poeductien,
ann —Ppretednt,
G) Assembly = te produce
Treat ue daughter Wlriu, Fuman 0b yy
rou they aus net deadly, apc cae
boutein SN
[Protease enryme. were the Hime Scole Us diy,
fe lepers
mciRes these cubyuses I NSaa eT ee
dead YY .Cmatute). a wdrrud ifjacks F human
2 lant meapind the fess
& Nore 1S HIV wore = 1460 rena
eae ; intel
Republic of Conge corte
mago’s US finsf 4 NEH——9 1905 —*1946 7 Soqutnautr,
d
, ey Thane bot sete
wee Eetyme Ee the (ork in locke — key methanstn| Te aeaine
— 4H) omprenair
a nelfini
e dhervunuly
note Lan ‘ " 3
See al bub tart greurng
Fie collate now tage grouting ceils too" =o haby:ete-
ek highly 4aS+ 4 4
a cures TOHY) Kyser omer
ee Se $(03|23.
srequlate @-P: a8
pentn - Angtotense 2 pcecangittensin cenverting ape
lin kctuney) etymes eee
ce ce ERO CHE Bi
Gractiud eaters —e SRBC.
ara ioteacinggen. uae sO Sar
= 4054) ,
“use th) sa eae ouerint
(rungs! __vasocenshritten
© Maosteren. ses See:
1c) Adveroh conter: 0 vaso ccostyictiCn.
veabsenphien 4 Nau
2 i ot
wat ks ‘ x
siale ene Apr At (Ant dllusating— Aknkcal AEMCON Stantfeance . nee
(BCE fahtoben. sand gat =>
Cagto pal,
speyg
eoapatl. rr a less poteat > . Sa
Llsno putt.
Yami psig]. |
ey Rentn Sobiottes, Sark |
;
,
Kem Rbren
®. Ragictensey Be L blockes.
Lewstan fe
Febicnwutan . i nie ei
—Qlucose
S
iN
breaking devin Frnobelism.
ee Semetning . Clubing together of
Gun 5 smaller pieces.
‘seolysis C euurconeo genesis).
Sr ea ys Cotycolyats),
|
a
.
wid cl rex”.
c
Eb, + cry fw Sytem Pa oO
cyan :
note oe,
O)qucese Rtseke is not vey active molecule 4
B eng ret
e
SS
|. pox? (Paaphoryjatiee) Con,
ys +» metabeliem
Gib F cautine, subs. tt ater’
until ther ase
A strategy OF mdycolgecs is ted
_prosphesyt geouye te qlucere
prncictic qiuuere (Calelore sugass), to frructere (ketese segey) .tera biocd pH
a wadtical
med €col
SoHE
steps
w catalyst = entsme
es ae
quease Hexokinase,
4
its werk is
qhesphate tte Jedna-
G-e-P-
@) Geo-P——> F-6P
woes ee
LOT
Cae we
: "
Ansine actde 4 Preaterns : oe
: $0
fee — 20AA Biogente
cect: [Ss Proteogente anrmino actd.
(Genetic code)
: KA amino aude (aminogroup
c S60 0 C
y cen).
wD De tts 5 @COOrr
Gunes pees eae —» at anda yetds = b [L
awed our body har J amine acti *— KPBA catbeolk
a® channel.
\ ~
| Ks sah ebitoy
Re
Out ob 20 amino acts.
NA AA bee ark lease | chtrral as
HN
wv oon (alacene)
ne C-cOrben
&
Ro
gard. :
Han Niver pe nn Ncoon Pimino ———* Pratedo
Soe : aud
\ —tye
Re re
HN Sty coon
Roe oss
ek 6
> ret: nal
i amite ie
bond.
(ftphde bond),
| <40 pephide
Y40 prateen. = looses Uneats SHY.
becomes mone & more complen.
How many amtnoactd tn ectapephide.? 3 amine acide
dtpepttde 2 amino actds
swetetn
ipeptide ——® ghutatyicne ( pieeme poraritemal x cide eftelt = Woe
Arieptide ie ata damage
ptide —» Acgectenuin IF - quaterthiane i algo given by docs )* rlyyetne Carty] G) ny
CH
oe
Branson Cae 1A) o-Cioon
ee
cect.
ae (valle Nhe
peycho .
AO
biosyntieses (anaball[em
Metbabiim | aivtne aut << PY ?
catabolism
Nha 2 ‘
RE _ tatabeticm, + Trnnc~amfnation Z
‘ooh, Cremoual 0€-nhs) ¥ J
C skeletan.
kyveb f
ThA ( Beebs cycle ) tnengy Wvedietién
eas Synthean .
chelettyel styntheais.
@ comes trem —» Deaniattion—» wnen ayele
40 emetic __—> Amncletic (etrectty te ents). (fches)
et “itt tecay (WHeote) (wena
aida: (way) TAT Ae
Untootatio).Currcogenesio) S cteps ¢
= psce HENS LEIT eucve |
TFroamaminalien >
ou e
Atpasctic (oy afro ‘)
Ubtep in mitachendee
vest Gin cytosol.
Qrithine |
yheutasele aedd
ud. art can ent
ues CEU
Lure cycle, (wre vycte?
stee t Franoaminatian
> Pas
Nh PON he ad cs Hp
coor CBN aks bon. * _ Goon o
e
andeo acd vete actel / Oe arated
Ta bet actd annine acid «
ae { edoh + toh’ | coon
eS, Uhh : ie 1 Enon
2 i Ue Urs 1 Ung
om : Sh 1 Loon ‘ toon
fo OOM ' exaloacetic | axpasctic
a Rete Glutaic —§ (qiutanctc attay! aed acted
akd. (KH kar) | (anifne actd) * (opad CAR)
(K rete auld) : ' '
t '
Reg, PL, a *GA)
Naas + 7 waeacycle
Coo
ancino
ackdy uy
Ve ASP
=e : am,
Use yycle . Yelog)2a
Bate total.
used.
o sy? ea)
orate acetic » z
acd.red + A + GAT?)
4
[ Cine Ki %
cascbonyt phosyhate ee mh tN
126)
ty
r eS USERS Ss he 2a Re aT Se ar Re an ga arn
ee mitochondria.
Ae
‘ Sit
Ornithine Re (du, bs =N-CHe Coon
ree Wh n ‘" «bas cectt
n.
Mey L citulline. (4),
Ad,
| ie
aaa Co
Coptocbetesection We | Weta,
L cckabolicm ray
(demydrectien)
LN (e hyduory phe Pyvuvate)
oh Dicxygenase AABN ALKA p
( Pecaxberylaticn)
wh rp -K Yost00h.
me Sexgenze,
ae nay = ALKApTEN ~
— On —t00h _otftedouy Ne
fbr: a
J 1.2 dKorygenate ra
tr seca cnae (HCN _isemenage ef seqorgly
(mateylacets peitnte De Famereacctnacesate,
onFunonit acid —_> TOR ae
Aceto acetic arid ——> Acetylccenzyme.
i | ong— i s-con |
Teyptophan ro) eee ee
Nhe ~~
co0H. }
Use
Gey ne |
Try. Mela
To Gh (s hyatory bry prophane)
AGes
i yo. Aint
oy st (ShyeKy Tryp tanccne)
reusnctrtnomitey) NY
aleekness), Cserate sone :
. Ne Acetyl ‘
prrcu Fergae.
Cus 7s : 4 ;
: | e-meth Ome transfers.
cn 0 mye 98 :
(newrve tran miter)
(metokenine)eae Uae grey eae aa /pa)tee
Gly colin (ene psiveliet = pyuuates , (eccw a muscles).
— tcp and bap mechan tac oid
Enzyme. : ;
Gane ae > BHP. ( the is an Iterchangabre | |recenstble
Phosphate ver". heen’ the Same tmeyme J
‘Somenate.
tyme Crud € ar Arming A Sy
4
= ce 0 AA ————>Some part of Ung
oN ricer ieee some part of flap.
—loop nae
only Fern ane
ible
Ca htne “verpenail 5
; us For vonueatiing won
CARD GS | Subrstaate into poreducts. aay cna
Cataliyte
more Yeeidues,
thio ‘ot canaeeare
ZEN, amine acide),
ere Sane fax
inne, Coacteeligfie ,
Supe Substrate entos Hhncugh fap io
SWostante WA go-to catalyhe ct,
| ceenponents io enayme NS
1) beta seeele, Sheets.
a hella.
“piece of belict.
TrtO St Phosphate isimtrare methanicm.
mre substrafe= bHAP » Peduck = gry colelehide
di hydreay acetone 3 pheaphate.
phosphate.DURP. GAP.
‘i ooh e
Ne eae,
ON.
2 ms eee eon
Nopod” Ne
0 FOsAbaconesgenesia. ( Corfeycle).
+ formation qluccse
* accu mandy in Liver.
Aefoe ackd metabelfem,
‘ StH r Nae
ae ee A
(SAH)
$- Adenouyt Homo ofefyomocyctety << —> methionine. TUE teen 5,
sn sth. Pate A
Att
®
SAM.
8 1 4 forctetn
denine SAY ge Beene
chy transfer Me RNA
rege: Crrettytactien) Lipid.
L
tes uutth aging,
ae ts gething methylated
iS - Net ge0d (here)
frokepe
mothylakien
.
eis. Hotty
netobolism ob Oytteln.
On SH
+ a \
oa oH Ne
Sonoogstuy hei an)
Oy Sesuine ON nae & Keto butyric.
Mie os MHy pe
‘WoO
cys teeth iSwrewasy
> Ane Sey
See eee of anti nw (prateote inhthiter)
Bye, degma
. coors en SAM JSAM
T - tramamenation 6
oa z meee + derostboxy tation * BCAA
Worea tse edeamtna ton
metatli £ signhth Ean catcnt
. Lv
a ceeud pregeic. eo uren cyle
an
e nuk p45
| RTREAT
Autophagy Ty [ut seln, pag <| te eat. pe
CO tasty eatira itselp. a oh
f gery,
metaloalismn ef Atantne sae
z a pegilttcse |
muse at ‘
e cell a.
heel ve ae Aiea
ate
£4 ee aay qymute aefd,
. = tania
~ Ryrunte acd A
i i ; Nascantatlen! =
Alanine. q>4~—_____™*> aatnite Meld
cou
HN 00H
(quecmte acld,)
|
|
XRG A + Aly
gmanatica scon
Ay ccoh \ x
¥ CoH
yr ore
a?
se® ONS
a NG COOH
(goutamtne)
8 ©
NB Nh
coon Keen
way (Asclewdne)
[ese +l,
é p
Secs oon
a¥ete valeste actd. dketo B me
valouie actd.
carboxylation ee
pared repay! wate, preptongl
coon eye coumryme
“BCAA metabeltem”
4 euential Av
+ Urtr hepatic metabcticm,
4104 }23,
oe.
soo Nees
y (ew
+N
’
SONS
a kate fcacay
HMG
eentyme
4
chalestrel synttest
Cescental ea
OF OU mene san
Cente — bloee
teed ane
fBP Rae
medttin,
ase
HAG trhtbetCe.
eS
oxenvastabnfactor =e = ubtamtna
oe Foorgante — nfnenals.
(big melecutes),
ve Fitke .
vere
© Feed se. cahtye.
Chet).
THE Cult Bay
Qetra hydro Fate acfd)
BG (coFacten)
ATP ( cofoctas),
(THe merhfentne
) tare
SAM
‘ } | metry tatien rar
SAM
Me,NF Tor \
ce
Ga hota, —~ homo oyster.
S4Cin roe
Uageect meinen)
anata bal 5Piven,
Auconeege nests. ea
Ly key enclecutte = oxaloacetate.
ngtt qlucancegcnecu = ghycelysis.
tnpey tne
ake en phosphors pyruvate
wr
erect all rx”, ae SAME,
cnly the abeve rex 1S povcutmable &
wighe Giff. BU? pyruvate & phe sPhe—pyrusaty
{ce cee
Bee oon fice OH) atte
=0 aj _ .
1 a Sie Oy Lysine
oS Orly
ra ryrwate
ddowiatd)
group. |
No 003
SS
Aa
: Prospne Enel pamuate.
; (er).
Hypoxia
q
4 WIF-4 hypoxia Tnduced
factor
Kinases Phocphoxdus Hae.
areFatty aed metabotion water solute = moolorde
aauy famatwe - : tat ane sdluale % water
Uipfd soluble — sotubte tw Ufaeld Mei Shae
atem
Tet fo water Car no hetevcatem) Me
hey HALE lary © chatn.
was actds = water scluble
eth Ge ty
pertien f tess and ¢ skeleten 4
Sas oa uty Mere «
men equal fer mur ane Fat 4 Cther nesuml— > rece dF tm ACcL aoe.
wy dfet-
roettootico! fox = fn =
© Sy ntea + cnetabelfin okextfne ee sae!
Fast actd seca
SS molecule.
codunated —— Ungateueated.
i cousiated rete cholutrol leuel more tran dfetny chotestce
“fat act “tH Seer #9 fotake.
Seas
nyu cera — Lon saunated
tal
emg 40 | :
4, nea
wing. + pretelnt ———> Spo pretetas,
™ commen Way +
Stone Lipids fo body.
| hues
rbosouption at
‘Tnteatine vibe
Peaidlireiec way tour deroity .
are tederge pe protecns
Coby le mfctions) (
pee aa
Bed inteimediate “MYr Beod sample wo TaRen fucem blead etc a hence we meaatise a
7 ty
oa “a
os Soe
Mocwlalxorwe) > ye a |
} fatale Se weak Hyg. vO deen! CH@h
3 oxy gycorat ie
Me cH on
a aeel CH-On.
ms Afpose = eneyrne
Lpose tuegger EN
(pactein Ketnawe A) chapateg OH the cite
band = dcld tree he
gt
orate = mere chance Sf dfabetes PON asic| >i
tece glyceral vemmtued apter the - Bigg
waosk ef Upwe ,glyceralenter —(@)_*# canbunatlve = +0 or copes
cnubchydsate cycle 4 fom DHAP ean 1 Sly Re AEE kas le.
weal re
a gucetiel tine cou :
Proeyiqdyertiel ~ gbuceneeg cneai,
° Rutty actd
faity aced oxidation.
= ae as
> Pf, Gnd. + OP) =
<< © Oty aulenylate.
+ HS—GA,
peas, 4
Cannet be akteched oe
fe.
e + ‘
need to be achiunted pl ere hme
ankds =~ ncbunuy weactiive &
Och deufyatives = reorkue.a i
Protetn : [pate > ne
WY Mav big encleewle : :
(2) &mPoungd Sh amino actol i
AA WA By — fit , peptide , anole bond. y go,
fy
GB) Nigh melas t : ii
9 maetg kt. HN Oo in —Neoon
—Q— =
(ene ——Ketructume}
——\ ‘> CRNA) y protefn =
\ ) vat —aneticnal uneyme -
Nas J
Central: Dogma.
uy
O Squmce Hhamlno ald
© _ Structure,
tnexe true maker
prctetnn dé fpesont
amongt themsclue,
Cenzyme ka tock £ key sty.)
tretefn = Fasqe noelecede < uranges Feey
Case StTupy
IQA) o \partiont cometea actor £ Cancer was Sucpected
fo sepanate protezn, Snctument war cused
anecey CORA t6ccue Ay
patient da heathy “HES
eha healthy patient
/ patil
{ dalton
Ss weight undt
—found a
} &tFerent: band. (53 KD)
tamed {t (p53)
avnaviien Foren
(pretetn),wohat ts councey
GC) me
cual a
cell CONS Cy le
is O
wabouy conbyolled
Prevess
UW duigion
ke epoutrr kas
Enzyme gene. deg.
sem the
753 —> pex® poutess Io
uncostvolledl,
a
APL cell dhuleter g v6,
urcantrouled =) UT oHey
(why?)
TSG,
Pechoncegene Hen Su ppseye
(dosent ae aeney
but when became
actiine, dim eee
cet eycle— fimo) ca fri
NKOY he (ty
Nee a Hamer Fea
theod feug!
counes at ‘Be
ee O3
EE (aie) ga cA
vrest Leuncesy
Jul Sa tse By
AO Ay x
chormeseme .
sete oF jB\P ke sox
the,
ma, a cont se canes be. ee
eh on 4 Brest atkal &
& soley (mas poten)
ce
& COU bantt jaa crahd hat i
and mati fan rake hae ) Heme, |
WE Ps? jo zyada tom, uw fnctument me olfkh raha tha.SHrucine
pf Trasey
Steandasy 2
te suasy
3D st
—Vecmplexty
Se
enlato An @ structures
- B shectu
H bandl .
o helte .
trcreases,
°
2-3 poictetn anpas me entangled ‘he jate hat:
NMB nucleas magnetic resenamne.
x
Row
> Cteelsran mecroscepe (Em)
Nn G6 PID 400 pF
Pretetn azlost
Ssolatieny J Purdhtcction,
: Stbte
Bete Fos ctectncarel itss Caner ¥thuctune
| Steps :
© “Binbltng ——+ Extrackion +3) énsifchment-—» (4): furdpication
| (snatteegy) (from celto eS Cirbtnee hugh
waa a 5; fractronation PWHE pretecn
(ereteind sents — Cserunaty d aes
* Wahi cheese. thi esctecn (Lem
nce) nen pxctecn
thera)
: © Structwrak
fusrtiicatien str. chasiactesu'sat® Unosackesitention,
a W Spectyoscepy |
© Onvem abe gorepy : v "4 “ ee
3D st. rhe
oe actd
(N +00
elteteren, terminal)
"
sume eshte
\ aan
MUA magnetic Spectresiopy
tag nanceMABRY <9 on be a
# ponte CO Hecue, ate
poh bo
a a ‘os
aay
wi ioulrc ie,
pant
sceal
cottua
geunga watey
» Set yoa tion
© gE den alerupe oy COLLULLAM menrbuane.
® NECA HOHE to de this
Steps
8) add COU do butter
Aechninges
© sontiaticn,
Nove fe
Mud PMGdiw ed 6
Rotel, _
OA they
Pod
Th nytip "ty
Eoruten ino uti
Mode Om, pai Fay
Hgut bral,
NeAUS.
w gut fHowa. vata
> poe bhatt
B aiseng gut
et batted?
“i
DALAL ER shock£ng > CELL Ro Frere base 4 tharutn
& Yomagenfcatlen, rep
(Ox heme dF nwbeer)
Bode mu.
BD eheraead methecls. 05 + Sargent a method.
Lysosymes ee 8):
fratease
——e Trten (datengud)
Creem tutep. 4
te occaty| @ enutchment /-fractienatten. iy -o a
Sepasat” € prcteta prem nen patefo on ee
Preteincan be puntfted buat ao @ basic Parrocopescbies
W Stee-/ moleallan coe pee UL Filtyatien. (Size exclusian)
ete oe sree — em ont OP geucbepes,
® Sclubtlitg . eer) ——> setting out
© Ang aan eee MS eee watt OF
_O7Sb eeForty ontd : mModt SiH : Weg
\ tony chain ¢ contatning fatty aefol. »
; \ body me hasunfr ebMtet.
ay energy ota
ay pare Of composition
MY es alae bode pak,
c \iu) this metabolism ig“
Ractd connected suit
TFA | # to'make them enter eettbedly Uiceue and) Cigale ine
wet acyl qlececie)
er ce
——> Fata + glycerol
A
AS) #N mure fat fF,
weubohydiiate Corn Cotte t
treteboldsiy Ov rnclaecilem. aby
\ #0 dfrect
ze petal Metabolism .
\ aduane a. \ .
\ + us-cch A) in
\ Shien) pt gf ads
coeneym A. ear oe dectuatives
— ie as ChesePuati ued are
s mone eactivue thay
ni ee Paley + Pr; autd:
nn + MS—CoA ele Gn + AMP.
Aeyt lof
Geuntting = a cancer Ruoteén.
Pemh Up = can wieach ce wut not dnetde tt
(From fscue)
C lost per eye)
Bein baiydation (1 cowiated fn) (7
1 oyelt
\ a Soria?
lec (eurn'e’). SD hyduolaa
DS “pee of uycle =D oxtlatren
i oun: > -), » triclysit-
otter! >
CoA
& wsorengancrgly PPS uti be weleued.diwefng 0
ode
(0, ewe body bat dendency ae |
eee '
% FAD EADL = canteg,. contiguoaten.
oe
* Mylo — atkene + worter.
+ '
Mucleuphdi (» torcben par On? jacga ar x'c’ me hfndncrnce
addcHen :
Aare
rex”,
F onfdatlon (3)
ee mee
Sabechab > ketene.
+ Awol,
NC) NAN NaBH Chips)
Hos CoA
__tyutoone part )aresage)
MS ® Nucleuphile oo of
ators fre, roe
i)
AAA Ep
=> GD me vat:
peerer
a ee
Ketone bodfex:__, wane etane func” qo wp.
Geretisen tex cLinhetor, :
matnly aetdte Ly nature.
4
ee mere teoumed > ee
|
|
.
|
P Injdsieay butyrate
J
Ou € me
LOHR that op erun
Je PY + here dEctuschane
on 1 Ue
Se erteive tic activi
€ 5
} Dinbetet —+ biecd OO Rushey
Ree — Le Log
Ar fs ohakkaa &
coma .Tusttication
Wy Poet pttation] Sabhng out. (rweten selubilr,)
@ pialysic. (sized based).
G) Centrifugation. (ansity based)
Wh dtenential centedpyation .
Ob fate Zone. ——u - ¢
Gil) fs0 pyente conta? Lag ation.
jhe 3
Bettas pate: AS a
oe Bi Ce or
centri tu= A y)
Scum ple gaticn eS
Based On Apecd
W nuumel cuntiifuge ——* upto 80,000 Sep.
2 ultra centriege ——* upto 0, 00,000 opr,
Lieronen tind conteftugation.
sample. different , fon differnt
Sooo. time.
sreen eetley
100 G Fence | 5 mtns —_, pettet get un
4000 G ] 10 mfns ———> nuctel +
15000 G /20 mtn, ———> maftochendefa eh
80,000 G | 4 hy ___y ploama _ fesf_csuapesnaterct bas
: - MEeMbIWLNe a Bie soluble: pure
prctefn.
demttey
lysed cella 5 a.)
lysed ce setae ay
miewsomer
O drmaity bued, def powtefu™haue same
+ ceootamira tion,OO
OD Rake Zone contd.
Conti Pig eat”,
Rs, sample
Vang pong,
4:2 G3 | Aeatity
vg time | dent.
Frute © aime
here emetty remains Const
-Fmass shecomer deff. F0Ctor,
MEBEG => atten SEH, CHeUy paliticle moue. Y9 ba tam
GW Isp
Hite canbulfugats ( Brteyant method) | quetitbrtien,
MERE ‘qct & pellet ene.
\ ee
\ denny Jeane .
Z v gradient -
high Ae
SBE AAA | ge teenation Put used
KK
et 9 Gke based) _-~ mdbtle. phase
a9 iS lasge wilt ceme out
(methenel [ we
Eixce & Smacler wit
ae aleehe\) cheromactugrreyshy
came out os, é ay
3} “gel. | ehare
| Saati bo Rie Ginsle ) Uy mebtte Rha
Rea ti
| \__fnteraction. os
ae posctite UU Z Ge gelaanrr
RE seposate pared on
re & not on
Mmolerdon vuefght .
LIU WI eluent ,
# Clutton
© vonge pascticle reecthe Seecthn cua jake
hou
# snaler pantiolee weedy me phos
We hats
= eee cn eeMednudoy. — (areat stor]
Fakty acid metebolicm.
catabolism — anacbol Snr.
sea dev syntheses .
y. & CoA
yeductisn of ketone bodiec. ge Joh
con Rae rae 3
‘ Veo / tHing pee
hea CoP / fe Hy
Ay? Sia . Ae Ys
ba o-% Gs 7 ~on
(mele rue 3 ere sae oe
ao =k group, -s-ten\ vided vid! — >
fs cledeun Acctoacctyl
mAb Roda ag (oA becom.
Wo/ hydseabyesy
oA
i)
“=O
U
ay
yon
Che
‘ vee
‘
Aeetese
Anaboltm
_———.
te Rex ane just opp astte-
° 8 §
gots! + he~rg bso! a Ret ~un-t_¢-r’
pcp = acy) carlo prctein,Eniyme Knetin ™ ltt .
aes
€
Soe Biocotalyst-
Soe er
G
tntermediater,
trans tin state.
HOME = biced cn tye Cf cnemtoat wroastiong trey nelp 10-
CYCLE
= (es) 4 len ShErP Hewte noco He 6%
Bees nt Eien { ane thovon Gs
Cd « MOLE le.
pel — ae eo = Ger +E]
ee
nae oe Step.
only tho step taker Hime, vest beeeme irreueuibly fost.
Exe Lisosume =