Study Design and Population

We retrospectively analyzed cross-sectional respiratory virus surveillance data collected during October 1, 2019–May 31, 2021, across 23 homeless shelters in King County, which includes the city of Seattle. As previously described, the Seattle Flu Study instituted active routine surveillance through staffed shelter kiosks (19,20). Study enrollment was open to residents >3 months of age reporting new or worsening cough alone or onset of >2 other acute respiratory illness symptoms in the previous 7 days, including subjective fever, sore throat, rhinorrhea, shortness of breath, headache, and myalgias. Symptom criteria also included diarrhea, rash, and ear pain or discharge for children <18 years of age. Persons who did not meet the symptom requirements were allowed to enroll and submit a nasal swab sample while asymptomatic up to once a month for shelter surveillance (i.e., inclusion criteria were broadened to allow a person to participate >1 time per month even if asymptomatic). Beginning April 1, 2020, eligibility expanded to all residents and staff regardless of symptoms as a result of the SARS-CoV-2 response (19). Nine shelters participated in the study, which included both participant and environmental testing, before the COVID-19 pandemic (October 2019–March 2020). An additional 14 shelters joined the study during April 2020–May 2021 but only for participant testing because of the need to shift resources toward identification and isolation of persons with SARS-CoV-2 infection.

We obtained written consent from participants >18 years of age or from a guardian for children <18 years of age; we obtained assent from participants 13–17 years of age. We offered $5 gift cards to compensate participants for their time. This study was approved by the Human Subjects Division of the University of Washington Institutional Review Board (approval no. STUDY00007800).

Data Collection

Study staff recruited participants at each shelter site 3–6 days per week. All participants completed a questionnaire on an electronic tablet and submitted a nasal swab specimen at each enrollment. Questionnaires were stored in Research Electronic Data Capture (https://www.project-redcap.org) and included information on current symptoms, shelter site, and demographics.

We collected respiratory specimens by using midturbinate sterile nylon flocked nasal swabs (FloqSwab; Copan Diagnostics) during October 1, 2019–July 22, 2020, and then subsequently during November 1, 2020–May 31, 2021. During July 22–November 1, 2020, we briefly used anterior nares swabs (US Cotton; SteriPack) because of supply change resource limitations. Given the spread of SARS-CoV-2, we changed the specimen collection protocol to study staff–supervised self-collected swab samples. We shared visual guides with participants before specimen collection to demonstrate self-swabbing.

We collected environmental samples weekly from 9 homeless shelters during November 20, 2019–April 10, 2020. We adapted collection methods described by Bailey et al. (21). With residents present, study staff swabbed a 10-cm² area of selected high-touch surfaces (e.g., kitchen counters, front desk, doors, and entrance and restroom doors) by using Berkshire Lab-Tip 125S swabs. We collected bioaerosol samples for 60 minutes in high-traffic areas by using an SKC QuickTake 30 air pump with ambient air pumped through Millipore filter papers. We stored all collected samples in Universal Transport Medium (Copan Diagnostics) and transported on ice.

Multiplex PCR Testing

We tested nasal swab and environmental samples by using a multiplex reverse transcription PCR platform (Open Array; ThermoFisher Scientific) for 28 viral respiratory pathogen targets, including pan-enterovirus, EV-D68, rhinovirus, influenza viruses (A, B, C), respiratory syncytial viruses (A and B), human parainfluenza viruses (1–4), human coronaviruses, human bocavirus, human parechovirus, human metapneumovirus, adenovirus, and SARS-CoV-2 (from specimens collected beginning January 1, 2020). We generated a relative cycle threshold (Ct) value for each result.

We identified positive or inconclusive enterovirus swabs by using PCR on either pan-enterovirus (ThermoFisher assay Vi06439631_s1) or EV-D68 (ThermoFisher assay Vi06439669_s1) targets and using a relative Ct value of <28 as provided by the manufacturer. Because the enterovirus probe can produce a false-positive test result on a sample with high rhinovirus amplification, laboratory staff reviewed all swab samples initially positive on enterovirus-specific primers and evaluated them on the basis of the degree of enterovirus amplification, enterovirus relative Ct values, and degree of rhinovirus amplification. Finally, we attempted sequencing on all positive or inconclusive enterovirus swabs identified by PCR to confirm enterovirus positivity and subtype.

Genomic Sequencing and Analysis

To identify viral species and genotypes present in enterovirus-positive swabs, we performed sequencing with enrichment for respiratory viruses using a commercially available panel of capture probes that covered multiple enteroviruses. We attempted whole-genome sequencing on all specimens and environmental samples that were positive or inconclusive for either the pan-enterovirus or EV-D68 targets. In our process, we converted extracted RNA to double-stranded cDNA, purified by bead cleanup, enzymatically fragmented, end-repaired, amplified, indexed, and purified again by using the QIAseq FX DNA Library Kit (QIAGEN, https://www.qiagen.com). We performed hybridization capture by using the QIAseq xHYB Viral Respiratory Panel (QIAGEN) after pooling libraries by sample relative Ct values. After overnight hybridization with biotinylated probes and subsequent washing to remove unbound fragments, we amplified the enriched libraries and purified them by using bead clean-up. We sequenced the resulting libraries on Illumina NovaSeq 6000 or NextSeq 2000 instruments by using a 2 × 150 read format. We generated consensus genomes by using a custom bioinformatic pipeline described previously (Appendix) (22).

We categorized specimens and samples as enterovirus-positive when they were positive or inconclusive by PCR and were sequence-confirmed as coxsackievirus or enterovirus. We considered any other sequence-confirmed viruses as enterovirus-negative and grouped them with swabs identified as other respiratory virus (ORV) –positive through PCR testing. We defined enterovirus unknown as any swabs that were initially identified as positive or inconclusive for pan-enterovirus or EV-D68 through PCR but were unable to be sequenced.

Computational Analysis

We analyzed demographic, symptom, respiratory virus, and environmental data descriptively by using R version 4.3.2 (The R Project for Statistical Computing). We linked multiple enrollments (i.e., encounters) from the same participant by name, date of birth, and sex, as previously described (18). We summarized enterovirus results by shelter type and highlighted shelter outbreaks with >5 enterovirus cases. We determined the frequency of enterovirus detection among shelter participants by dividing the number of sequence-confirmed positive specimens by the total number of participant encounters overall and during viral circulation. We used an intercept-only Poisson regression model fitted using generalized estimating equations to obtain robust SE estimates and 95% CIs, accounting for clustering by shelter site. We used NextStrain software to process consensus genomes and to generate and visualize phylogenetic trees (23). We calculated bootstrap values using IQ-TREE version 1.6.12 (24). In addition to the consensus genomes generated for this study (Appendix Table 1), we downloaded and included in our analyses full-length CVA21 and EV-D68 genomes available from GenBank.

Participant Surveillance

During October 1, 2019–May 31, 2021, we collected 14,464 nasal swab specimens from 3,281 unique participants (22% staff, 78% residents) across 23 homeless shelters (Appendix Table 2, Figure 1). Swabs from children <18 years of age constituted 14% of all specimens collected.

PCR testing identified 83 participant specimens on either the pan-enterovirus (n = 73) or EV-D68 (n = 46) PCR targets. Upon sequencing, we found 55 confirmed enterovirus-positive specimens among 47 symptomatic shelter residents during October 3, 2019–March 6, 2020 (Figures 1, 2; Appendix Tables 2–4). We detected no enterovirus-positive specimens among shelter staff eligible to participate during April 2020–May 2021. Compared with episodes with enterovirus-negative specimens, episodes with enterovirus-positive specimens were associated with an older median age and being male, being a current tobacco smoker, experiencing chronic homelessness (>1 year), and having underlying conditions (Appendix Table 2). Although the difference in age was attenuated when comparing specimens restricted to enrollment during October 2019–March 2020, other differences remained even after the expansion of eligibility during April 2020–May 2021 (Appendix Tables 3, 4).

We identified cases of CVA21 (n = 39) and EV-D68 (n = 5) among adults and CVA6 (n = 2) and CVA4 (n = 1) among children. Six residents tested CVA21-positive at 2 different timepoints, with a median of 9 days between positive tests (range 2–26 days). Two EV-D68–positive residents tested positive at 2 different timepoints (median 14 days, range 2–26 days). Four coxsackievirus-positive residents had rhinovirus co-detected.

The median age of CVA21-positive persons was 47 years (range 23–72 years). Most (90%) were male; 41% identified as White and 21% as Black/African-American (Appendix Table 2). The most commonly reported signs or symptoms of CVA21 infection included runny nose (85%) and cough (67%) (Figure 3; Appendix Tables 2, 5). Among the 39 unique persons with CVA21 infections, 51% (n = 20) reported a symptom or symptoms that prevented daily activity (Figure 3; Appendix Tables 5, 6). Half of the persons with CVA21 or EV-D68 indicated that their illness affected socialization, followed by those indicating that their illness affected their ability to take care of themselves or their family (36%), exercise (32%), and work (30%). Although 4 CVA21-positive persons sought care at a doctor’s office or an urgent care setting, most (69% of persons with CVA21, 80% of persons with EV-D68) did not seek any medical care (Appendix Table 6).

Overall, CVA21 detection among all participant encounters was 0.3% (45/14,464 [95% CI 0.2%–0.5%]) during October 2019–May 2021 and 3.0% (45/1,485 [95% CI 1.9%–4.8%]) during viral circulation during October 2019–February 2020 (Figure 1; Appendix Table 7). Although we detected CVA21 across 7 shelter sites (Figure 1; Appendix Table 7), most cases occurred in outbreaks at 2 large adult shelters: 19 at mixed-gender shelter L with adults >18 years of age (October 10, 2019–January 10, 2020) (Figure 2; Video 1) and 10 at all-male shelter M with older adults >50 years of age (October 3, 2019–January 27, 2019) (Figure 2; Video 2).

Environmental Surveillance

Of 812 environmental swabs, we identified 18 on the pan-enterovirus (n = 8) or EV-D68 (n = 17) PCR targets, and we sequence-confirmed 6 as CVA21 (n = 5) or EV-D68 (n = 1) (Appendix Tables 8, 9, Figure 2). Detection of enterovirus-positive environmental swabs occurred during November 20, 2019–March 12, 2020, across 3 shelters, all which also had resident cases detected. Most CVA21-positive environmental samples (n = 3) were detected at shelter L, which had the largest outbreak of cases among residents (Video 1). Despite having 10 unique CVA21-positive cases and 4 EV-D68-positive cases among its residents, the older adult male shelter (M) did not have any environmental samples that tested enterovirus-positive (Video 2). Surfaces where CVA21 was detected included bathroom doors and the front desk. We detected only 1 sequence-confirmed EV-D68–positive environmental sample from a bathroom door. We detected other viruses in environmental samples through PCR targets more frequently than enteroviruses; the highest rate of detection was for rhinovirus on children’s playroom table (36%, n = 10), front desk (25%, n = 23), and restroom doors (23%, n = 31) (Appendix Table 9). Environmental surfaces tested consisted of plastic, Formica, or metal (Appendix Figure 3). None of the 99 bioaerosol samples tested were positive for enterovirus or another respiratory virus (Appendix Table 9).

Genomic Analysis

Because positive environmental samples may represent mixtures of viruses from multiple shelter residents or staff, we focused our genomic analysis on sequenced species from unique participants (Appendix Table 8). We collected all EV-D68 genomes from 5 unique participants during a 3-week period (October 10–31, 2019) from 2 shelters, L (n = 1) and M (n = 4). These formed a single cluster among 1,032 publicly available EV-D68 genomes downloaded from GenBank (Figure 4); specimens from shelter M did not cluster separately from the specimen from shelter L. All 5 genomes were of EV-D68 clade A2 and among the genomes from GenBank were most closely related to 2 genomes (GenBank accession nos. OR230417 and OR230423) collected in the United States in 2020. The environmental EV-D68 sample also was clade A2 but did not cluster with the participant specimens among the GenBank genomes (Appendix Figure 4).

All CVA21 genomes from 39 unique participants across 7 shelters formed a single phylogenetic cluster among 29 publicly available CVA21 genomes downloaded from GenBank (Figure 5, panel A). The study genomes fall within CVA21 cluster I (9,25) and are mostly closely related to a genome collected in Nepal in 2017 (GenBank accession no. MZ396299). We observed some clustering by shelter (Figure 5, panels B, C) and instances of identical genomes at the same shelter. The mean pairwise genetic distances between specimens from the same shelter were lower than those from different shelters; however, this difference was not statistically significant (p = 0.0927 by analysis of variance) (Appendix Table 10). We observed no shelters with >2 sequenced participant specimens where all shelter genomes formed a single phylogenetic cluster and, among sequence clusters with >90% bootstrap support, we observed both single and multiple shelter groups. We also noted instances where >1 viral lineage of CVA21 appeared to be circulating at the same shelter at the same time (e.g., shelter M in October 2019). Finally, we observed an association between time of specimen collection and viral genotype given that all 6 specimens collected in 2020 formed a single cluster. Phylogenetic trees including the 5 sequenced environmental CVA21 samples (Appendix Figure 5) illustrate that 4 of 5 environmental samples were closely related to other specimens from the same shelter. The other sample from shelter L was not closely related to any other sequenced shelter specimens and, given its position in the tree, might represent a mixture of viral genotypes observed among the CVA21 shelter specimens.

We visualized the single sequenced CVA4 specimen in a phylogenetic tree among publicly available CVA4 genomes (Appendix Figure 6); the most closely related GenBank genome was collected in Tennessee in April 2015 (GenBank accession no. KY271949). The 2 sequenced CVA6 specimens cluster together among publicly available CVA6 genomes (Appendix Figure 7). The GenBank genome most closely related to these strains was collected in France in 2018 (GenBank accession no. MT814570).