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#pychiptools

Installation

Clone this repository and then:

$ cd pychiptools/
$ python setup.py install --user

This will install the scripts in the pychiptools/scripts directory. For more information on the individual scripts, use the --help command after each script.

##Core Pipeline

  • pychip_align.py -> Wrapper for FASTQC, cutadapt and bowtie1/bowtie2 sequence aligner.
  • pychip_ucsc.py -> Takes a sam file and converts it to a UCSC formatted bigWig
  • pychip_peak_call.py -> Peak calling using MACS2 and SICER

##Additional Tools

  • pychip_peak_anno.py -> Peak annotation using chipseqanno, homer and also a custom annotation
  • pychip_motifs.py -> Denovo motif discovery implemented using MEME and HOMER. Also motif search using FIMO
  • pychip_diff_bind.py -> Differential binding implemented using a variety of programs.
  • pychip_download.py -> Tool for downloading samples from GEO

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Pipeline for ChIP-seq analysis

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