djstub project provides a dj64/dj32-compatible and go32-compatible stub that support COFF, PE and ELF payloads. Its primary target is dj64dev suite, but it can also work with djgpp-built executables or plain COFF and ELF files.

• It can stubify or re-stub COFF and PE executables. See djstubify.
• It can link the ELF binaries into a dj64/dj32 executables. See djlink.
• It can strip the debug info from dj64/dj32 executables. See djstrip.

$ djstubify -i comcom64.exe
dj64 file format
Overlay 0 (i386/ELF DOS payload) at 23368, size 30548
Overlay 1 (x86_64/ELF host payload) at 53916, size 87048
Overlay 2 (x86_64/ELF host debug info) at 140964, size 174936
Overlay name: comcom64.exe
Stub version: 4
Stub flags: 0x0b07

$ djlink -d dosemu_hello.exe.dbg libtmp.so -n hello.exe -o hello.exe tmp.elf

These examples are documented in a dj64dev project.