Python-based program for the identification of effective siRNAs and the evaluation of effective dsRNAs.

Linux, Mac or a WSL-capable Windows version

bash install.sh

The installer will install miniforge into ~/RNAival_Dependencies and install dependencies through conda. The installer tries to create a desktop entry in usr/share/applications, which requires root-user priviliges, but is not critical for usage. On Windows/WSL, prefix the commands with "wsl" or switch to a WSL terminal beforehand.

bash run.sh

• Create a new project
• Select sequencing libraries (fastq.gz)
• Select target sequences (embl,fasta,fna.gz)
• Select targets and evaluation types for libraries
• Run the pipeline