Add abi and custom adapters options to porechop_abi#591
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sofstam
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Apr 3, 2025
jfy133
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Apr 4, 2025
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
jfy133
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Apr 10, 2025
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@nf-core-bot fix linting |
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@nf-core-bot fix linting |
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@nf-core-bot fix linting |
jfy133
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Apr 11, 2025
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
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@nf-core-bot fix linting |
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One correction to usage.md and slight improvement to parameter doc but otherwise LGTM
| Both tools support length filtering of reads and can be tuned with `--shortread_qc_minlength`. Performing length filtering can be useful to remove short (often low sequencing complexity) sequences that result in unspecific classification and therefore slow down runtime during classification/profiling, with minimal gain. | ||
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| There are currently two options for long-read Oxford Nanopore processing: [`porechop`](https://github.com/rrwick/Porechop), [`porechop_abi`](https://github.com/bonsai-team/Porechop_ABI). | ||
| You can enable on the `abi` option by turning on `--longread_qc_predictadapters` to predict adapters directly from the reads. |
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I think this needs it own subsection/eader as it is tool specific guidance (the rest seems to be generic), and also --longread_qc_predictadpaters doesn't tell it to use porechop_abi does it?
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You are right, it does not explain that -longread_qc_predictadpaters uses porechop_abi. Making the docs more clear.
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
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| You can also turn off clipping and only perform paired-end merging, if requested. This can be useful when processing data downloaded from the ENA, SRA, or DDBJ (`--shortread_qc_skipadaptertrim`). | ||
| Both tools support length filtering of reads and can be tuned with `--shortread_qc_minlength`. Performing length filtering can be useful to remove short (often low sequencing complexity) sequences that result in unspecific classification and therefore slow down runtime during classification/profiling, with minimal gain. | ||
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| ###### Long reads |
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| ###### Long reads | |
| ##### Long reads |
jfy133
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Apr 15, 2025
jfy133
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Apr 15, 2025
jfy133
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Thank @LilyAnderssonLee @sofstam ! |
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Add
params.longread_qc_predictadaptersandparams.longread_qc_adapterlistasext.argsfor the modulePORECHOP_ABIsuggested by @AlexHoratio #589PR checklist
nf-core pipelines lint).nextflow run . -profile test,docker --outdir <OUTDIR>).nextflow run . -profile debug,test,docker --outdir <OUTDIR>).docs/usage.mdis updated.docs/output.mdis updated.CHANGELOG.mdis updated.README.mdis updated (including new tool citations and authors/contributors).