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eQTL_analysis

Purpose: Expression quantitative trait loci (eQTL) identification for a population of 33 healthy canines.

  1. Genotypes preprocessing
  • I was provided with two tsv files including the genotype data from Axiom without their supporting files, therefore I was not able to use bcftools affy2vcf to create the vcf file so I wrote my own function to convert these files to vcf files (See genotyping_code.R)
  • All data was then lifted over to canfam4 (code)
  • The vcf file was then imputed using Beagle (code)
  1. Phenotypes preprocessing
  2. Covariates
  3. Running tensorQTL

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