Standalone multi-channel OME-TIFF viewer with a built-in web UI.

Point it at a file or a folder and open your browser.

• What it is
• Supported formats
• Installation
• Quick start
• Command-line reference
• Environment variables
• Configuration file
• Filename conventions
• Programmatic use
• Troubleshooting
• Development
• Docker migration
• License
• Acknowledgements

TissueViewer is a zero-config viewer for high-resolution multi-channel microscopy images. It serves deep-zoom tiles over HTTP and ships with a pre-built OpenSeadragon front-end that lets you colourise channels, adjust gains and windowing, and save per-slide settings.

• OME-TIFF (.ome.tif, .ome.tiff) — any bit depth, any number of channels, with or without a built-in pyramid.

Support for additional formats (plain TIFF, OME-Zarr) is planned as pluggable handlers; see src/tissueviewer/formats/ for the registry.

pip install tissueviewer

Or from a source checkout:

git clone https://github.com/davidvi/tissueviewer.git
cd tissueviewer
pip install .

Requires Python 3.10 or later. On macOS / Linux / Windows.

Open a single file:

tissueviewer /path/to/slide.ome.tiff

Browse a folder of slides (root folder becomes the public location):

tissueviewer /path/to/slides

Browse a nested tree of folders:

tissueviewer /path/to/slides --recursive --open

Use a config file:

tissueviewer --config config.yaml

Check every file in a directory can be opened, then exit:

tissueviewer /path/to/slides --validate

usage: tissueviewer [TARGET] [options]

positional:
  TARGET                Path to an .ome.tif(f) file or a directory of them.

options:
  -r, --recursive       Include nested subdirectories.
  -c, --config FILE     YAML configuration file.
  -p, --port N          Listen port (default 8000).
  -H, --host HOST       Bind address (default 127.0.0.1).
  -o, --open            Open the default web browser after start.
  -w, --watch           Rescan TARGET on filesystem changes.
      --validate        Verify every OME-TIFF, then exit.
      --no-save         Disable writing .sample.json sidecars.
      --log-level LEVEL DEBUG | INFO | WARNING | ERROR  (default INFO).
  -V, --version         Show version and exit.
  -h, --help            Show help and exit.

TARGET is optional if either --config is given or TV_SLIDE_DIR is set.

Kept for backward compatibility with the Docker deployment. CLI and config values take precedence.

# config.yaml
data_dir: /srv/tissue-viewer/slides
recursive: true
host: 0.0.0.0
port: 8000
save_enabled: false
log_level: INFO
colors:
  - red
  - green
  - blue

tissueviewer --config config.yaml

Precedence (highest wins): CLI flags → environment variables → YAML → built-in defaults.

All recognized slide files end in .ome.tif or .ome.tiff (case-insensitive).

For a slide named patient42.ome.tiff, two sidecar files may appear next to it:

Delete them freely to reset.

The web UI groups slides into locations. The mapping:

• Single-file mode → the file lives under the public location.
• Directory mode (no --recursive) → files at the root → public; every immediate subdirectory → its own location named after the subdirectory.
• Directory mode with --recursive → files at the root still go to public; files in nested subdirectories are grouped under a slash-joined location path like 2024/batch_A.

create_app builds a regular FastAPI instance, suitable for any ASGI server (uvicorn, hypercorn, daphne).

from tissueviewer import Config, create_app

app = create_app(Config(data_dir="/srv/slides", recursive=True))

uvicorn my_module:app --host 0.0.0.0 --port 8000

• ModuleNotFoundError: imagecodecs — install imagecodecs or reinstall tissueviewer; many OME-TIFFs use LZW/JPEG/Zstd compression.
• Port already in use — tissueviewer --port 8001.
• Blank tiles / wrong colours — delete the slide's .sample.json to reset settings, then reload.
• Apple Silicon wheels — imagecodecs and opencv-python-headless ship arm64 wheels; no local build required.
• Zarr 2 vs 3 — both are supported. The pin is zarr>=2.15,<4.
• OpenCV GUI warning — tissueviewer depends on opencv-python-headless; a warning about cv2.imshow not being available is normal and harmless.

See CONTRIBUTING.md for local setup, how to rebuild the Vue UI, and how to cut a release.

TissueViewer used to ship as a Docker image with a Java-based bioformats2raw conversion pipeline. That mode is gone in 0.2; see MIGRATION.md for guidance.

Creative Commons Attribution-NonCommercial 4.0 International — see LICENSE.md.

TissueViewer uses OpenSeadragon for high-performance tiled image visualisation.