multiPrime is a mismatch-tolerant minimal primer set design tool for large and diverse sequences (e.g. Virus). Here is a web-based version (test: http://multiPrime.cn)

Acconeer Exploration Tool

The SpeciesPrimer pipeline is intended to help researchers finding specific primer pairs for the detection and quantification of bacterial species in complex ecosystems.

Design degenerated primers on highly variable alignments for full genome sequencing or qPCR. Specifically developed for viruses.

MFEprimer-2.0: A fast thermodynamics-based program for checking PCR primer specificity

A PCR primer tool for DNA assembly flows

Clermont PCR method In-Silico

This project will give real analysis of Option chain along with PCR Graph and MaxPain gragh

A tool in order to accurately remove primer sequences from NGS reads in an amplicon experiment

Overlapped primer design for entire coding sequence of the gene with specificity check

CRISPR/Cas-directed HDR genome editing suite: finds+scores gRNA targets, generates donor DNAs, & produces optimal cPCR primer designs.

龙花菜，以公主连结Re:Dive和龙图为主要服务的QQ机器人。

In Silico PCR for Python3

A lightweight Python package to perform in silico PCR

This is a workflow to develop multiplex assay from singleplex data in PCR

Oligopool Calculator - Automated design and analysis of oligopool libraries

Bioinformatics CNV Detection with Random Forest Model

Real-time PCR data viewer (Python 3 GUI with wxPython)

Variant Primer Design

Clermont PCR method In-Silico