Scribd
Upload
13 views1 page

Adenosine Desaminasa (Ada) : ADVIA 2400

The document provides guidelines for validating reagents on an ADVIA 2400 instrument for measuring adenosine deaminase (ADA), including reagent preparation instructions, instrument settings, analytical parameters, calculation method settings, and standards settings.

Uploaded by

michel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
13 views1 page

Adenosine Desaminasa (Ada) : ADVIA 2400

The document provides guidelines for validating reagents on an ADVIA 2400 instrument for measuring adenosine deaminase (ADA), including reagent preparation instructions, instrument settings, analytical parameters, calculation method settings, and standards settings.

Uploaded by

michel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 1

ADENOSINE DESAMINASA (ADA)

Adenosine Glutamate-dehydrogenase

The application parameters comprised here constitute a guide to facilitate the validation of our reagents by the
instrument. It is advisable to validate the use when there is any change in software or reagent versions.

Instrument: ADVIA 2400


Reagent preparation
Working Reagent: Reconstitute the content of reagent B vial with the contents of reagent A vial. Mix gently. Stable for 30 days at 2-8 ºC.

Instrument settings

ANALYTICAL PARAMETERS Reanalysis conditions Multi-Standards setting


Analytical Conditions Serum reac. smp. vol. (µ) 3.00 Formula Linear correction Axis conv. No convert.
R1 volume 100.00 Serum dilut. method (µ) None Points 1
R2 volume 0.00 Serum reac. smp. vol. (d) 3.00
R3 volume 0.00 Serum dilut. method (d) None FV MEAN
R4 volume 0.00
R1 diluent vol. 0.00 Standards setting 1 * ...
R2 diluent vol. 0.00 BLK H 9.9999
R3 diluent vol. 0.00 BLK L -9.9999
R4 diluent vol. 0.00 STD H 9.9999
Serum reac. s. vol. 25.0 STD L -9.9999
Serum dil. method Standard FV *
Abnml. (serum) H 99999
Reaction time 10 min. Abnml. (serum) L -9999
Reagent 1 stir. Weak
Reagent 2 stir. Weak
Reagent 3 stir. Weak Calculation method setting Reaction rate method
Reagent 4 stir. Weak M-DET. P. l 19 Prozone Cycle 3
M-DET. P. m 22 Prozone form. None Factor 3.0
Sub-analy. conditions M-DET. P. n 36 Prozone limit 9.999 Reac. Type Dec.
Name ADA S-DET. P. p 0 Prozone judge Upper limit E2 corre. Not do
Digits 1 S-DET. P. r 0 Judge limit 9.999 Blank (µ) 9.9999
M-wave. L. 340 nm M-DET. P. m. 0 Blank (d) -9.999
S-wave. L. Check D.P.I. 0 M-DET. P. n. 0 Sample (µ) 9.9999
Analy. mthd. RRA Limit value 0.003 S-DET. P. p. 0 Sample (d) -9.999
Calc. mthd STD Variance 10.0 S-DET. P. r. 0
Qualit. judg. Not do Endpoint method
Re. Absorb (µ) 9.9999
Re. Absorb (d) -9.999

Vers. 1606

You might also like