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DavidRach/README.md

Currently teaching:

  • Cytometry in R: A Course for Beginners Basically the course I wish had been available back in 2020 when I got thrown into the R deep end without knowing how to swim. Intentionally aimed at those with flow cytometry expertise, but no-to-limited R skills.

Currently working on in R:

  • Luciernaga Tools for quality control and signature evaluation of SFC unmixing controls.
  • Coereba How heterogenous are the cells within your SFC panels.
  • InstrumentQC An automated dashboard for monitoring Cytek and BD InstrumentQC data.
  • InstrumentQC Install How-To Current method of implementing an InstrumentQC website for other core facilities.
  • CytometryQC Nearing completion, should allow for one-click installation of InstrumentQC websites.

Upcoming Conference Talks and Posters

  • Bioc2026 : Cytometry in R: A free weekly course for flow cytometrist with no-to-little coding experience. Accepted - Oral

Looking for the code from one of our previous papers, talk or poster?

  • CYTO2026: Being Everything, Everywhere, All at Once: Open-Source Automation for Situational Awareness in SRLs. Slides Code Abstract

  • CYTO2026: A semi-supervised pipeline for a comprehensive and scalable analysis of immune heterogeneity in human samples. Slides Code Abstract

  • CYTO2026: Cytometry in R: A free weekly course for coding beginners. Poster Code Abstract

  • ABRF2026: Complex Data Analysis - No Cells Left Behind: Wrangling the Full Biological Discovery Potential out of Spectral Flow Cytometry (SFC) datasets. Slides Code

  • Frontiers in Immunology Cord Blood Innate-like T cells Paper Code

  • CYTO2025:“Well, how bright does it need to be?”: Investigating the interplay of fluorescent signature and brightness in single-color unmixing controls. Slides Code Abstract

  • CYTO2025:“Well, how bright does it need to be?”: Investigating the interplay of fluorescent signature and brightness in single-color unmixing controls. Poster Code Abstract

  • CYTO2025:“Are these autofluorescences in the room with us right now?” Quantifying impact of autofluorescence variation on unmixing. Poster Code Abstract

  • CYTO2025: “Wait, when was QC last run???” Evaluating MFI drift after morning QC and its impact on unmixing. Poster Code Abstract

  • GammaDelta2023: Vγ9Vδ2 T cell responses in HIV-exposed Uninfected (HEU) Infants Poster Code Abstract

  • CYTO2023: Spectral Flow Cytometry Analysis of Innate-like T cell responses in Malawian HIV-exposed Uninfected (HEU) Infants Poster Code Abstract

  • ASTMH2021: Innate-like T cell responses in HIV exposed uninfected (HEU) Malawian infants Poster Code Abstract

I'm graduating Wednesday, June 17, 2026 and looking for my next adventure:

Fun fact: Ultramarathon's are really just food festivals. They are generally run on trails out in nature, there's no shame in hiking the hill, and the aid stations every three miles have food that is to die for. And if that wasn't enough to make you want to sign up, some even give out hand-crafted coffee mugs to finishers.

Pinned Loading

  1. Luciernaga Luciernaga Public

    Tools for the evaluation of Spectral Flow Cytometry (SFC) unmixing controls

    R 4 3

  2. Coereba Coereba Public

    Dichotomized Clustering for Spectral Flow Cytometry

    R 1 2

  3. UMGCCCFCSR/CytometryInR UMGCCCFCSR/CytometryInR Public

    Course Website

    R 389 607

  4. CordBloodILT CordBloodILT Public

    Repository for the "Cord Blood Innate-like T cell responses in neonates born to healthy women and women living with HIV" paper, containing code and additional resources

    HTML

  5. UMGCCFCSS/InstrumentQC UMGCCFCSS/InstrumentQC Public

    Visualized QC data for the Cytek instruments.

    JavaScript 1 9

  6. InstrumentQC_Install InstrumentQC_Install Public

    Installation how-to for the InstrumentQC dashboard

    SCSS 4 4