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dotBlast

Tool to generate dot plot from BLAST nuleotide output

Requirements:

  • BLAST+ (tested v2.6.0+)
  • Python (tested v3.6.7)

Usage:

dotBlast.py <reference_file> <queries_file> <eval>

reference_file: FASTA file with reference sequence
queries_file: FASTA file with query sequences
eval: Maximum E-value to consider in BLAST alignment

Output:

queries_vs_ref.txt

Each line represents the x or y component of each alignment in the queries file order.

<query_1_x>
<query_1_y>
<query_2_x>
<query_2_y>
...

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Tool to generate dot plots from BLAST output

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