Threshold independent detection and localization of diffraction-limited spots.
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Updated
Apr 8, 2026 - Python
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diffraction-limited-spots
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Fluospotter is an end-to-end pipeline designed for nuclei segmentation and puncta detection in fluorescence microscopy images.
deep-learning segmentation cell-segmentation cell-biology u-net 3d-volume microscopy-images diffraction-limited-spots spot-detection
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Oct 2, 2024 - Python
region-of-interest band-pass-filter statistical-significance-test metrics-evaluation diffraction-limited-spots scale-space-feature-detection total-internal-reflection-fluorescence-micscopy epifluorescence-microscopy fluorescent-speckle-microscopy low-signal-to-noise-ratio subresolution-cluster-of-fluorophores speckle-modulation-analysis imaging-technique-evaluation imaging-quality-metrics gaussian-spatial-frequencies-filtering 2d-convolution-operator image-noise-reduction bessel-function-to-gaussian-fitting vinculin latrunculin
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Nov 26, 2023 - MATLAB
inflection-point bessel-function intensity-curvature jacobian-matrix diffraction-limited-spots difference-of-gaussian scale-space-feature-detection resolution-limit low-snr fluorescent-speckle-microscopy point-spread-function-fitting objective-function-optimization generalized-sparrow-limit detection-success-rate change-of-sign-2nd-derivative airy-disk-modeling subresolution-cluster-fluorophores feature-fusion-due-to-noise computationally-inexpensive-detection
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Aug 7, 2025 - MATLAB
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