Independent project I undertook to perform a full ChIP-Seq analysis of the transcription factor Nanog in Zebrafish embryos.

A front-end GUI to map NGS DNA sequencing data using HISAT backend tool. This software offers robust seamless queueing of the mapping operations along with parameter memory for quick and easy customization.

A tool for generating and processing nucleotide conversion tables from HISAT-3N alignment results.

These are tutorials on a subset of tools available for processing raw RNAseq data. This if for HISAT2_SAMtools_Stringtie_gffcompare_ballgown pipeline or HISAT2_SAMtools_Stringtie_PrepDEanalysis.py_DESeq2 pipeline

A pre-processing (alignment) pipeline based on hisat2

Analysis of .fastq ChIP-seq datasets

Build Docker container for HISAT2 and (optionally) convert to Apptainer/Singularity.

Bioinformatics Programming - Transcriptome Assembly

RNA-Seq pipelines that use HISAT2, Kallisto, Salmon, DESeq and Sleuth.

Investigating unmapped reads within next generation sequencing data will provide additional information regarding the source of the trace microbial reads.

rna-seq pipeline for drosophila melanogaster: qc, trimming, alignment, and read counting (81.7% alignment, ~50m bp processed)

Comprehensive bioinformatics workflow for RNA-seq quality control, trimming, alignment, and gene quantification. Automates the complete pipeline from raw FASTQ to gene expression counts using FastQC, FASTP, HISAT2, and featureCounts.

Proof of concept of a RNA-Seq pipeline from reads to count matrix (including quality control) with Nextflow and additional example RNA-Seq analysis in R

Repository for bulk RNA-seq analyses (from undergrad 2nd-3rd year) – Reorganized

Evaluating HISAT2, Salmon and Kallisto alignment using Checkpoint Blockade treated CT26 Experimental Data

Cell-surface glycoRNA-based cancer detection.

Guide for aligning reads to a reference genome with HISAT2.

Single-end RNA-seq pipeline: FastQC → Trimmomatic → HISAT2 → featureCounts. 93.98% alignment rate.

This project uses an workflow pipeline to generate map and assemble RNAseq reads to a reference genome. Furthermore, we generate counts data and identify differentially expressed genes from 2 conditions.

Scripts to index and align Bovine genome with HISAT2