www.PDHcenter.com PDH Course M143 www.PDHonline.
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(2) The specific requirements for ventilation and filtration to dilute and remove contamination in
the form of odor, airborne microorganisms and viruses, and hazardous chemical and
radioactive substance;
(3) The different temperature and humidity requirements for various areas; and
(4) The design sophistication needed to permit accurate control of environmental conditions."
Isolation rooms and isolation anterooms with appropriate ventilation-pressure relationships are
the primary means used to prevent the spread of airborne viruses in the hospital environment."
The isolation rooms can be classified in three basic categories:
• Negative Pressure Isolation Rooms
• Positive Pressure Isolation Rooms
• Multi-level Biohazard Laboratories
1) Negative Pressure Isolation Rooms maintain a flow of air into the room, thus keeping
contaminants and pathogens from reaching surrounding areas. Because of potential litigation
concern, the exhaust air is also normally filtered through HEPA filters to ensure
contamination free release to environment. Generally the infectious areas are maintained
negative pressure with respect to adjacent spaces. A simple example of negative pressure
isolation room is in health industry for Tuberculosis (TB) Rooms. 6 to 12 air changes are
recommended from TB rooms. Supply air to the room, is also filtered. Ultraviolet Germicidal
Irradiation (UVGI), commonly known as UV light, may be used to augment HEPA filters, but
cannot be used in place of HEPA filters, as their effectiveness on airstreams is limited.
2) Positive Pressure Isolation Rooms maintain a flow of air out of the room, thus protecting the
patient from possible contaminants and pathogens, which might otherwise enter. The most
common application today is HIV Rooms and rooms for patients with other types of
immunodeficiency. For such patients it is critically important to prevent the ingress of any
pathogens, including even common fungi and bacteria, which may be harmless to healthy
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