DDX5
DDX5DEAD 박스 단백질 5 또는 RNA 헬리케이스 p68로도 알려진 ATP 의존성 RNA 헬리케이스 DDX5는 사람에서 DDX5 [5]유전자에 의해 암호화되는 효소이다.
기능.
보존 모티브 Asp-Glu-Ala-Asp(DEAD)를 특징으로 하는 DEAD 박스 단백질은 추정 RNA 헬리케이스이다.이들은 번역 개시, 핵 및 미토콘드리아 스플라이싱, 리보솜 및 스플라이세솜 조립과 같은 RNA 2차 구조의 변화를 포함하는 많은 세포 과정에 관여한다.그들의 분포 패턴에 근거하여, 이 과의 일부 구성원들은 배아 발생, 정자 형성,[6] 그리고 세포 성장과 분열에 관여하는 것으로 여겨진다.이 유전자는 RNA의존성 ATP효소인 DEAD 박스 단백질과 증식 관련 핵항원을 암호화하며, 특히 시미안 바이러스 40 종양 항원과 반응한다.이 유전자는 13개의 엑손으로 구성되며, 여러 개의 인트론 서열을 포함하는 대체 스플라이스된 트랜시션이 검출되었지만, 이러한 트랜시션에 의해 코드된 아이소폼은 [5]확인되지 않았다.
상호 작용
DDX5는 다음 제품과 상호 작용하는 것으로 나타났습니다.
- AKAP8,[7]
- DDX17 (p72)[8]
- DHX9(RNA 헬리케이스 A),[9]
- 에스트로겐 수용체 알파,[10][11]
- 피브릴린[12]
- HDAC1,[13]
- 핵수용체 공동활성화제 [14]1,
- 핵수용체 공동활성화제 [14]2
- 핵수용체 공동활성화제 [14]3,
- p53,[15]
- CTCF[16]
레퍼런스
- ^ a b c GRCh38: 앙상블 릴리즈 89: ENSG00000108654 - 앙상블, 2017년 5월
- ^ a b c GRCm38: 앙상블 릴리즈 89: ENSMUSG000020719 - 앙상블, 2017년 5월
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ a b "Entrez Gene: DDX5 DEAD (Asp-Glu-Ala-Asp) box polypeptide 5".
- ^ Legrand, JMD; Chan, AL; La, HM; Rossello, FJ; Änkö, ML; Fuller-Pace, FV; Hobbs, RM (23 May 2019). "DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia". Nature Communications. 10 (1): 2278. Bibcode:2019NatCo..10.2278L. doi:10.1038/s41467-019-09972-7. PMC 6533336. PMID 31123254.
- ^ Akileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM (May 2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi:10.1074/jbc.M101171200. PMID 11279182.
- ^ Ogilvie VC, Wilson BJ, Nicol SM, Morrice NA, Saunders LR, Barber GN, Fuller-Pace FV (March 2003). "The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells". Nucleic Acids Res. 31 (5): 1470–80. doi:10.1093/nar/gkg236. PMC 149829. PMID 12595555.
- ^ Wilson BJ, Giguère V (2007). "Identification of novel pathway partners of p68 and p72 RNA helicases through Oncomine meta-analysis". BMC Genomics. 8: 419. doi:10.1186/1471-2164-8-419. PMC 3225811. PMID 18005418.
- ^ Endoh H, Maruyama K, Masuhiro Y, Kobayashi Y, Goto M, Tai H, Yanagisawa J, Metzger D, Hashimoto S, Kato S (August 1999). "Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor α". Mol. Cell. Biol. 19 (8): 5363–72. doi:10.1128/MCB.19.8.5363. PMC 84379. PMID 10409727.
- ^ Rossow KL, Janknecht R (January 2003). "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300". Oncogene. 22 (1): 151–6. doi:10.1038/sj.onc.1206067. PMID 12527917.
- ^ Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (June 2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase". Exp. Cell Res. 257 (2): 272–80. doi:10.1006/excr.2000.4886. PMID 10837141.
- ^ Wilson BJ, Bates GJ, Nicol SM, Gregory DJ, Perkins ND, Fuller-Pace FV (August 2004). "The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner". BMC Mol. Biol. 5: 11. doi:10.1186/1471-2199-5-11. PMC 514542. PMID 15298701.
- ^ a b c Watanabe M, Yanagisawa J, Kitagawa H, Takeyama K, Ogawa S, Arao Y, Suzawa M, Kobayashi Y, Yano T, Yoshikawa H, Masuhiro Y, Kato S (March 2001). "A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor α coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA". EMBO J. 20 (6): 1341–52. doi:10.1093/emboj/20.6.1341. PMC 145523. PMID 11250900.
- ^ Bates GJ, Nicol SM, Wilson BJ, Jacobs AM, Bourdon JC, Wardrop J, Gregory DJ, Lane DP, Perkins ND, Fuller-Pace FV (February 2005). "The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor". EMBO J. 24 (3): 543–53. doi:10.1038/sj.emboj.7600550. PMC 548656. PMID 15660129.
- ^ Yao H, Brick K, Evrard Y, Xiao T, Camerini-Otero RD, Felsenfeld G (2010). "Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA". Genes Dev. 24 (22): 2543–2555. doi:10.1101/gad.1967810. PMC 2975930. PMID 20966046.
추가 정보
- Iggo RD, Jamieson DJ, MacNeill SA, et al. (1991). "p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts". Mol. Cell. Biol. 11 (3): 1326–33. doi:10.1128/MCB.11.3.1326. PMC 369403. PMID 1996094.
- Hloch P, Schiedner G, Stahl H (1990). "Complete cDNA sequence of the human p68 protein". Nucleic Acids Res. 18 (10): 3045. doi:10.1093/nar/18.10.3045. PMC 330836. PMID 2349099.
- Ford MJ, Anton IA, Lane DP (1988). "Nuclear protein with sequence homology to translation initiation factor eIF-4A". Nature. 332 (6166): 736–8. Bibcode:1988Natur.332..736F. doi:10.1038/332736a0. PMID 2451786. S2CID 4351448.
- Iggo R, Gough A, Xu W, et al. (1989). "Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction". Proc. Natl. Acad. Sci. U.S.A. 86 (16): 6211–4. Bibcode:1989PNAS...86.6211I. doi:10.1073/pnas.86.16.6211. PMC 297807. PMID 2762324.
- Buelt MK, Glidden BJ, Storm DR (1994). "Regulation of p68 RNA helicase by calmodulin and protein kinase C". J. Biol. Chem. 269 (47): 29367–70. doi:10.1016/S0021-9258(18)43885-9. PMID 7525583.
- Brody LC, Abel KJ, Castilla LH, et al. (1995). "Construction of a transcription map surrounding the BRCA1 locus of human chromosome 17". Genomics. 25 (1): 238–47. doi:10.1016/0888-7543(95)80131-5. PMID 7774924.
- Endoh H, Maruyama K, Masuhiro Y, et al. (1999). "Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor α". Mol. Cell. Biol. 19 (8): 5363–72. doi:10.1128/MCB.19.8.5363. PMC 84379. PMID 10409727.
- Rössler OG, Hloch P, Schütz N, et al. (2000). "Structure and expression of the human p68 RNA helicase gene". Nucleic Acids Res. 28 (4): 932–9. doi:10.1093/nar/28.4.932. PMC 102575. PMID 10648785.
- Nicol SM, Causevic M, Prescott AR, Fuller-Pace FV (2000). "The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase". Exp. Cell Res. 257 (2): 272–80. doi:10.1006/excr.2000.4886. PMID 10837141.
- Watanabe M, Yanagisawa J, Kitagawa H, et al. (2001). "A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor α coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA". EMBO J. 20 (6): 1341–52. doi:10.1093/emboj/20.6.1341. PMC 145523. PMID 11250900.
- Akileswaran L, Taraska JW, Sayer JA, et al. (2001). "A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase". J. Biol. Chem. 276 (20): 17448–54. doi:10.1074/jbc.M101171200. PMID 11279182.
- Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033.
- Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
- Liu ZR (2002). "p68 RNA Helicase Is an Essential Human Splicing Factor That Acts at the U1 snRNA-5′ Splice Site Duplex". Mol. Cell. Biol. 22 (15): 5443–50. doi:10.1128/MCB.22.15.5443-5450.2002. PMC 133941. PMID 12101238.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Rossow KL, Janknecht R (2003). "Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300". Oncogene. 22 (1): 151–6. doi:10.1038/sj.onc.1206067. PMID 12527917.
- Guil S, Gattoni R, Carrascal M, et al. (2003). "Roles of hnRNP A1, SR Proteins, and p68 Helicase in c-H-ras Alternative Splicing Regulation". Mol. Cell. Biol. 23 (8): 2927–41. doi:10.1128/MCB.23.8.2927-2941.2003. PMC 152554. PMID 12665590.
- Fujita T, Kobayashi Y, Wada O, et al. (2003). "Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells". J. Biol. Chem. 278 (29): 26704–14. doi:10.1074/jbc.M301031200. PMID 12738788.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.