In the Phantom Hourglass manga by Akira Himekawa, Jolene first appears when Link, Ciela, and Linebeck learn of Oshus's true identity and set sail to stop Bellum. Jolene boards their ship and attacks Link, mistaking him for Linebeck.[18] Jolene soon discovers his hiding place in a crate. She threatens him and he asks her what he did to her. Link rushes in and interrupts them, saying that they need Linebeck and asking her to delay her revenge.[19] Jolene suddenly notices Tetra and questions Link how she was turned to stone. Link informs her of his journey, while Linebeck uses the distraction to sneak out of the crate. Jolene glances at him out of the corner of her eye and attempts to stab him. Link asks what her relationship to Linebeck is, commenting that they "seem connected."[20] Jolene then tells him her past. Three years ago, on a small pirate ship, Jolene was attacked by a monster. Linebeck defeated the monster, though, according to him, it was an accident.[21] Jolene then asks him to join her, but soon, Linebeck snuck off alone with a treasure. At this point, Linebeck returns the treasure, which happens to be three-year-old squid jerky. Jolene tells him that the real reason she is so mad is that he left her. Ciela scolds Linebeck, telling him that he should try to be more sensitive to Jolene's feelings.[22] Jolene kicks Linebeck, telling him that she does not care about him. Linebeck apologizes, but Bellum appears and possesses him. Jolene leaves to find Zauz to forge the Phantom Sword. After Jolene leaves, Bellum tells Link that people who are feeling either friendship or love have more life force, and that is why it possessed Linebeck. After Link defeats Bellumbeck, Jolene arrives and gives Link the Phantom Sword. Link then rushes off to defeat Bellum.
Jolene is last seen directly before Link and Tetra are sent back to their world.