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79 stars written in C
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A versatile pairwise aligner for genomic and spliced nucleotide sequences

C 2,166 460 Updated Apr 25, 2026

MMseqs2: ultra fast and sensitive search and clustering suite

C 2,040 283 Updated Apr 16, 2026

Burrow-Wheeler Aligner for short-read alignment (see minimap2 for long-read alignment)

C 1,731 579 Updated Mar 22, 2025

Toolkit for processing sequences in FASTA/Q formats

C 1,539 328 Updated Jun 1, 2025

De novo assembler for single molecule sequencing reads using repeat graphs

C 921 179 Updated Apr 3, 2026

Near-optimal RNA-Seq quantification

C 758 181 Updated Mar 16, 2026

BWK awk modified for biological data

C 639 117 Updated Aug 11, 2022

Prodigal Gene Prediction Software

C 538 94 Updated Jan 23, 2024

Redbean: A fuzzy Bruijn graph approach to long noisy reads assembly

C 530 93 Updated Sep 27, 2023

HMMER: biological sequence analysis using profile HMMs

C 409 90 Updated Feb 16, 2026

Align proteins to genomes with splicing and frameshift

C 401 23 Updated Jan 5, 2026

Fast and accurate de novo assembler for long reads

C 400 54 Updated May 10, 2024

Long read / genome alignment software

C 316 31 Updated Dec 16, 2025

haplotypic duplication identification tool

C 283 23 Updated Oct 30, 2025

UCSC Genome Browser source. "beta" is released version / "master" is testing.

C 263 99 Updated Apr 30, 2026

Tools for manipulating sequence graphs in the GFA and rGFA formats

C 249 22 Updated Dec 16, 2025

Program for aligning DNA sequences, a pairwise aligner.

C 245 41 Updated Mar 27, 2026

Fast and accurately polish the genome generated by long reads.

C 243 30 Updated Jan 9, 2025

Detecting sites of genomic enrichment

C 201 30 Updated May 8, 2023

Implementation of the Pairwise Sequentially Markovian Coalescent (PSMC) model

C 189 61 Updated Nov 17, 2025

UCSC command line bioinformatic utilities

C 189 96 Updated Jul 31, 2024

Yet another k-mer analyzer

C 169 12 Updated Dec 30, 2025

Nanopore data assembler

C 168 24 Updated Mar 27, 2022

Ultra-fast de novo assembler using long noisy reads

C 136 31 Updated Feb 24, 2021

TAD calling, phase imputation, 3D modeling and more for diploid single-cell Hi-C (Dip-C) and general Hi-C

C 117 17 Updated Jan 8, 2026

Bioinformatics tools for dealing with Multiple Alignment Format (MAF) files.

C 110 32 Updated Jun 17, 2022

PHAST

C 83 31 Updated Apr 23, 2026

The MARVEL assembler

C 80 22 Updated Jul 7, 2023
C 75 84 Updated Apr 23, 2020
C 71 18 Updated Apr 28, 2020
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